GZMB / Granzyme B Antibody (N-Terminus)
Rabbit Polyclonal Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application ![]()
| IHC-P |
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Primary Accession | P10144 |
Reactivity | Human, Mouse, Rat |
Host | Rabbit |
Clonality | Polyclonal |
Calculated MW | 28kDa |
Dilution | IHC-P (1:100) |
Gene ID | 3002 |
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Other Names | Granzyme B, 3.4.21.79, C11, CTLA-1, Cathepsin G-like 1, CTSGL1, Cytotoxic T-lymphocyte proteinase 2, Lymphocyte protease, Fragmentin-2, Granzyme-2, Human lymphocyte protein, HLP, SECT, T-cell serine protease 1-3E, GZMB, CGL1, CSPB, CTLA1, GRB |
Reconstitution & Storage | +4°C or -20°C, Avoid repeated freezing and thawing. |
Precautions | GZMB / Granzyme B Antibody (N-Terminus) is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | GZMB {ECO:0000303|PubMed:32188940, ECO:0000312|HGNC:HGNC:4709} |
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Function | Abundant protease in the cytosolic granules of cytotoxic T- cells and NK-cells which activates caspase-independent pyroptosis when delivered into the target cell through the immunological synapse (PubMed:1985927, PubMed:3262682, PubMed:3263427). It cleaves after Asp (PubMed:1985927, PubMed:8258716). Once delivered into the target cell, acts by catalyzing cleavage of gasdermin-E (GSDME), releasing the pore- forming moiety of GSDME, thereby triggering pyroptosis and target cell death (PubMed:31953257, PubMed:32188940). Seems to be linked to an activation cascade of caspases (aspartate-specific cysteine proteases) responsible for apoptosis execution. Cleaves caspase-3, -9 and -10 (CASP3, CASP9 and CASP10, respectively) to give rise to active enzymes mediating apoptosis (PubMed:9852092). Cleaves and activates CASP7 in response to bacterial infection, promoting plasma membrane repair (By similarity). |
Cellular Location | Secreted. Cytolytic granule. Note=Delivered into the target cell by perforin (PubMed:20038786). |
Volume | 250 µl |

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Provided below are standard protocols that you may find useful for product applications.
Background
This enzyme is necessary for target cell lysis in cell- mediated immune responses. It cleaves after Asp. Seems to be linked to an activation cascade of caspases (aspartate-specific cysteine proteases) responsible for apoptosis execution. Cleaves caspase-3, -7, -9 and 10 to give rise to active enzymes mediating apoptosis.
References
Schmid J.,et al.J. Immunol. 139:250-256(1987).
Caputo A.,et al.J. Biol. Chem. 263:6363-6369(1988).
Trapani J.A.,et al.Proc. Natl. Acad. Sci. U.S.A. 85:6924-6928(1988).
Klein J.L.,et al.Genomics 5:110-117(1989).
Caputo A.,et al.J. Immunol. 145:737-744(1990).

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