DUT / DUTPase Antibody (clone 1C9)
Mouse Monoclonal Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| WB, IHC-P, IF, E |
---|---|
Primary Accession | P33316 |
Reactivity | Human |
Host | Mouse |
Clonality | Monoclonal |
Clone Names | 1C9 |
Calculated MW | 27kDa |
Dilution | IF (10 µg/ml), IHC-P (5 µg/ml), |
Gene ID | 1854 |
---|---|
Other Names | Deoxyuridine 5'-triphosphate nucleotidohydrolase, mitochondrial, dUTPase, 3.6.1.23, dUTP pyrophosphatase, DUT |
Reconstitution & Storage | Store at -20°C. Aliquot to avoid freeze/thaw cycles. |
Precautions | DUT / DUTPase Antibody (clone 1C9) is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | DUT |
---|---|
Function | Catalyzes the cleavage of 2'-deoxyuridine 5'-triphosphate (dUTP) into 2'-deoxyuridine 5'-monophosphate (dUMP) and inorganic pyrophosphate and through its action efficiently prevents uracil misincorporation into DNA and at the same time provides dUMP, the substrate for de novo thymidylate biosynthesis (PubMed:17880943, PubMed:8631816, PubMed:8805593). Inhibits peroxisome proliferator- activated receptor (PPAR) activity by binding of its N-terminal to PPAR, preventing the latter's dimerization with retinoid X receptor (By similarity). Essential for embryonic development (By similarity). |
Cellular Location | [Isoform 2]: Nucleus |
Tissue Location | Found in a variety of tissues. Isoform 3 expression is constitutive, while isoform 2 expression correlates with the onset of DNA replication (at protein level). Isoform 2 degradation coincides with the cessation of nuclear DNA replication (at protein level) |
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Provided below are standard protocols that you may find useful for product applications.
Background
This enzyme is involved in nucleotide metabolism: it produces dUMP, the immediate precursor of thymidine nucleotides and it decreases the intracellular concentration of dUTP so that uracil cannot be incorporated into DNA.
References
Ladner R.D.,et al.J. Biol. Chem. 271:7745-7751(1996).
Cohen D.,et al.Genomics 40:213-215(1997).
Pearlman R.E.,et al.Submitted (OCT-1997) to the EMBL/GenBank/DDBJ databases.
Ladner R.D.,et al.J. Biol. Chem. 272:19072-19080(1997).
Chano T.,et al.Submitted (SEP-2000) to the EMBL/GenBank/DDBJ databases.
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