APH1A / APH-1 Antibody (Internal)
Goat Polyclonal Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| WB, IHC-P, E |
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Primary Accession | Q96BI3 |
Reactivity | Human, Mouse, Rat, Rabbit, Hamster, Monkey, Pig, Horse, Bovine, Dog |
Host | Goat |
Clonality | Polyclonal |
Calculated MW | 29kDa |
Dilution | ELISA (1:128000), IHC-P (5 µg/ml), WB (0.05-0.1 µg/ml) |
Gene ID | 51107 |
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Other Names | Gamma-secretase subunit APH-1A, APH-1a, Aph-1alpha, Presenilin-stabilization factor, APH1A, PSF |
Target/Specificity | Human APH1A. This antibody is expected to recognize isoform 1 (NP_001071096.1) and isoform 2 (NP_057106.2). |
Reconstitution & Storage | Store at -20°C. Minimize freezing and thawing. |
Precautions | APH1A / APH-1 Antibody (Internal) is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | APH1A |
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Synonyms | PSF |
Function | Non-catalytic subunit of the gamma-secretase complex, an endoprotease complex that catalyzes the intramembrane cleavage of integral membrane proteins such as Notch receptors and APP (amyloid- beta precursor protein) (PubMed:12297508, PubMed:12522139, PubMed:12679784, PubMed:12763021, PubMed:25043039, PubMed:26280335, PubMed:30598546, PubMed:30630874). Required for normal gamma-secretase assembly (PubMed:12471034, PubMed:12522139, PubMed:12763021, PubMed:19369254). The gamma-secretase complex plays a role in Notch and Wnt signaling cascades and regulation of downstream processes via its role in processing key regulatory proteins, and by regulating cytosolic CTNNB1 levels (Probable). |
Cellular Location | Endoplasmic reticulum membrane; Multi-pass membrane protein. Golgi apparatus, Golgi stack membrane; Multi-pass membrane protein. Note=Predominantly located in the endoplasmic reticulum and in the cis-Golgi |
Tissue Location | Widely expressed. Expressed in leukocytes, lung, placenta, small intestine, liver, kidney, spleen thymus, skeletal muscle, heart and brain. Isoform 1 and isoform 2 are nearly expressed at the same level. |
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Provided below are standard protocols that you may find useful for product applications.
Background
Essential subunit of the gamma-secretase complex, an endoprotease complex that catalyzes the intramembrane cleavage of integral proteins such as Notch receptors and APP (beta-amyloid precursor protein). It probably represents a stabilizing cofactor for the presenilin homodimer that promotes the formation of a stable complex.
References
Lee S.-F.,et al.J. Biol. Chem. 277:45013-45019(2002).
Lee H.-J.,et al.Submitted (MAY-2002) to the EMBL/GenBank/DDBJ databases.
Lai C.-H.,et al.Genome Res. 10:703-713(2000).
Clark H.F.,et al.Genome Res. 13:2265-2270(2003).
Ota T.,et al.Nat. Genet. 36:40-45(2004).
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