SMAD6 Antibody (clone 2A6)
Mouse Monoclonal Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| WB, IHC-P, E |
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Primary Accession | O43541 |
Reactivity | Human |
Host | Mouse |
Clonality | Monoclonal |
Clone Names | 2A6 |
Calculated MW | 53kDa |
Dilution | IHC-P (5 µg/ml) |
Gene ID | 4091 |
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Other Names | Mothers against decapentaplegic homolog 6, MAD homolog 6, Mothers against DPP homolog 6, SMAD family member 6, SMAD 6, Smad6, hSMAD6, SMAD6, MADH6 |
Reconstitution & Storage | Store at -20°C. Aliquot to avoid freeze/thaw cycles. |
Precautions | SMAD6 Antibody (clone 2A6) is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | SMAD6 |
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Synonyms | MADH6 |
Function | Transforming growth factor-beta superfamily receptors signaling occurs through the Smad family of intracellular mediators. SMAD6 is an inhibitory Smad (i-Smad) that negatively regulates signaling downstream of type I transforming growth factor-beta (PubMed:9436979, PubMed:16951688, PubMed:22275001, PubMed:9759503, PubMed:10647776, PubMed:10708948, PubMed:10708949, PubMed:30848080). Acts as a mediator of TGF-beta and BMP anti-inflammatory activities. Suppresses IL1R-TLR signaling through its direct interaction with PEL1, preventing NF-kappa-B activation, nuclear transport and NF-kappa-B- mediated expression of pro-inflammatory genes (PubMed:16951688). Blocks the BMP-SMAD1 signaling pathway by competing with SMAD4 for receptor- activated SMAD1-binding (PubMed:9436979, PubMed:30848080). Binds to regulatory elements in target promoter regions (PubMed:16491121). |
Cellular Location | Nucleus. |
Tissue Location | [Isoform B]: Expressed in the brain, heart, ovary, peripheral blood leukocytes, small intestine, spleen, thymus, bone marrow, fetal liver and lymph nodes. |
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Background
Acts as a mediator of TGF-beta and BMP antiflammatory activity. Suppresses IL1R-TLR signaling through its direct interaction with PEL1, preventing NF-kappa-B activation, nuclear transport and NF-kappa-B-mediated expression of proinflammatory genes. May block the BMP-SMAD1 signaling pathway by competing with SMAD4 for receptor-activated SMAD1-binding. Binds to regulatory elements in target promoter regions.
References
Riggins G.J.,et al.Nat. Genet. 13:347-349(1996).
Hata A.,et al.Genes Dev. 12:186-197(1998).
Afrakhte M.,et al.Biochem. Biophys. Res. Commun. 249:505-511(1998).
Hagiwara K.,et al.Submitted (JAN-1998) to the EMBL/GenBank/DDBJ databases.
Konrad L.,et al.Submitted (NOV-2007) to the EMBL/GenBank/DDBJ databases.
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