CDC42BPA / MRCK Antibody (N-Terminus)
Goat Polyclonal Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| IHC-P, E |
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Primary Accession | Q5VT25 |
Reactivity | Human, Mouse, Rat, Rabbit, Monkey, Horse, Bovine |
Host | Goat |
Clonality | Polyclonal |
Calculated MW | 197kDa |
Dilution | ELISA (1:16000), IHC-P (3.75 µg/ml), |
Gene ID | 8476 |
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Other Names | Serine/threonine-protein kinase MRCK alpha, 2.7.11.1, CDC42-binding protein kinase alpha, DMPK-like alpha, Myotonic dystrophy kinase-related CDC42-binding kinase alpha, MRCK alpha, Myotonic dystrophy protein kinase-like alpha, CDC42BPA {ECO:0000312|EMBL:CAH71336.1}, KIAA0451 |
Target/Specificity | Human CDC42BPA / MRCK. This antibody is expected to recognise both reported Human isoforms (NP_055641.3 and NP_003598.2). |
Reconstitution & Storage | Store at -20°C. Minimize freezing and thawing. |
Precautions | CDC42BPA / MRCK Antibody (N-Terminus) is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | CDC42BPA {ECO:0000312|EMBL:CAH71336.1} |
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Synonyms | KIAA0451 |
Function | Serine/threonine-protein kinase which is an important downstream effector of CDC42 and plays a role in the regulation of cytoskeleton reorganization and cell migration (PubMed:15723050, PubMed:9418861, PubMed:9092543). Regulates actin cytoskeletal reorganization via phosphorylation of PPP1R12C and MYL9/MLC2 (PubMed:21457715). In concert with MYO18A and LURAP1, is involved in modulating lamellar actomyosin retrograde flow that is crucial to cell protrusion and migration (PubMed:18854160). Phosphorylates: PPP1R12A, LIMK1 and LIMK2 (PubMed:11340065, PubMed:11399775). May play a role in TFRC-mediated iron uptake (PubMed:20188707). In concert with FAM89B/LRAP25 mediates the targeting of LIMK1 to the lamellipodium resulting in its activation and subsequent phosphorylation of CFL1 which is important for lamellipodial F-actin regulation (By similarity). Triggers the formation of an extrusion apical actin ring required for epithelial extrusion of apoptotic cells (PubMed:29162624). |
Cellular Location | Cytoplasm {ECO:0000250|UniProtKB:O54874}. Cell projection, lamellipodium {ECO:0000250|UniProtKB:Q3UU96}. Note=Displays a dispersed punctate distribution and concentrates along the cell periphery, especially at the leading edge and cell-cell junction. This concentration is PH-domain dependent. Localizes in the lamellipodium in a FAM89B/LRAP25-dependent manner. {ECO:0000250|UniProtKB:O54874, ECO:0000250|UniProtKB:Q3UU96} |
Tissue Location | Abundant in the heart, brain, skeletal muscle, kidney, and pancreas, with little or no expression in the lung and liver. |
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Background
Serine/threonine-protein kinase which is an important downstream effector of CDC42 and plays a role in the regulation of cytoskeleton reorganization and cell migration. Regulates actin cytoskeletal reorganization via phosphorylation of PPP1R12C and MYL9/MLC2. In concert with MYO18A and LURAP1, is involved in modulating lamellar actomyosin retrograde flow that is crucial to cell protrusion and migration. Phosphorylates: PPP1R12A, LIMK1 and LIMK2. May play a role in TFRC-mediated iron uptake.
References
Wilkinson S.,et al.Nat. Cell Biol. 7:255-261(2005).
Totoki Y.,et al.Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases.
Bechtel S.,et al.BMC Genomics 8:399-399(2007).
Gregory S.G.,et al.Nature 441:315-321(2006).
Zhao Y.,et al.J. Biol. Chem. 272:10013-10020(1997).
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