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ABH2 / ALKBH2 Antibody (C-Terminus)
Rabbit Polyclonal Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, IHC-P, E |
|---|---|
| Primary Accession | Q6NS38 |
| Reactivity | Human, Mouse, Rat |
| Host | Rabbit |
| Clonality | Polyclonal |
| Calculated MW | 29kDa |
| Dilution | IHC-P (5 µg/ml), WB (1-2 µg/ml), |
| Gene ID | 121642 |
|---|---|
| Other Names | Alpha-ketoglutarate-dependent dioxygenase alkB homolog 2, 1.14.11.33, Alkylated DNA repair protein alkB homolog 2, DNA oxidative demethylase ALKBH2, Oxy DC1, ALKBH2, ABH2 |
| Target/Specificity | Human ALKBH2 |
| Reconstitution & Storage | Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles. Store undiluted. |
| Precautions | ABH2 / ALKBH2 Antibody (C-Terminus) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | ALKBH2 |
|---|---|
| Synonyms | ABH2 {ECO:0000303|PubMed:16174769} |
| Function | Dioxygenase that repairs alkylated nucleic acid bases by direct reversal oxidative dealkylation. Can process both double- stranded (ds) and single-stranded (ss) DNA substrates, with a strong preference for dsDNA (PubMed:12486230, PubMed:12594517, PubMed:16174769, PubMed:20714506, PubMed:25797601, PubMed:23972994). Uses molecular oxygen, 2-oxoglutarate and iron as cofactors to oxidize the alkyl groups that are subsequently released as aldehydes, regenerating the undamaged bases. Probes the base pair stability, locates a weakened base pair and flips the damaged base to accommodate the lesion in its active site for efficient catalysis (PubMed:18432238, PubMed:22659876). Repairs monoalkylated bases, specifically N1- methyladenine and N3-methylcytosine, as well as higher order alkyl adducts such as bases modified with exocyclic bridged adducts known as etheno adducts including 1,N6-ethenoadenine, 3,N4-ethenocytosine and 1,N2-ethenoguanine (PubMed:12486230, PubMed:12594517, PubMed:16174769, PubMed:20714506, PubMed:25797601, PubMed:23972994, PubMed:26408825). Acts as a gatekeeper of genomic integrity under alkylation stress. Efficiently repairs alkylated lesions in ribosomal DNA (rDNA). These lesions can cause ss- and dsDNA strand breaks that severely impair rDNA transcription (PubMed:23972994). In a response mechanism to DNA damage, associates with PCNA at replication forks to repair alkylated adducts prior to replication (PubMed:19736315, PubMed:26408825). |
| Cellular Location | Nucleus. Nucleus, nucleolus. Nucleus, nucleoplasm. Note=Relocates to the replication foci during S-phase. |
| Tissue Location | Detected in colon, small intestine, ovary, testis, prostate, skeletal muscle, heart, liver and urinary bladder |
| Volume | 50 µl |
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
Background
Dioxygenase that repairs alkylated DNA and RNA containing 1-methyladenine and 3-methylcytosine by oxidative demethylation. Can also repair alkylated DNA containing 1- ethenoadenine (in vitro). Has strong preference for double- stranded DNA. Has low efficiency with single-stranded substrates. Requires molecular oxygen, alpha-ketoglutarate and iron.
References
Lin Y.,et al.Submitted (SEP-2004) to the EMBL/GenBank/DDBJ databases.
Tsujikawa K.,et al.J. Cell. Mol. Med. 11:1105-1116(2007).
Scherer S.E.,et al.Nature 440:346-351(2006).
Duncan T.,et al.Proc. Natl. Acad. Sci. U.S.A. 99:16660-16665(2002).
Aas P.A.,et al.Nature 421:859-863(2003).
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