ISLET-1 / ISL1 Antibody (clone 1H9)
Mouse Monoclonal Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| WB, IHC-P, IF, E |
---|---|
Primary Accession | P61371 |
Reactivity | Human |
Host | Mouse |
Clonality | Monoclonal |
Clone Names | 1H9 |
Calculated MW | 39kDa |
Dilution | ELISA (1:10000), IF (1:200-1:1000), IHC-P (1:200), WB (1:500-1:2000) , |
Gene ID | 3670 |
---|---|
Other Names | Insulin gene enhancer protein ISL-1, Islet-1, ISL1 |
Target/Specificity | Human ISL1 |
Reconstitution & Storage | Long term: -20°C; Short term: +4°C. Avoid repeat freeze-thaw cycles. |
Precautions | ISLET-1 / ISL1 Antibody (clone 1H9) is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | ISL1 |
---|---|
Function | DNA-binding transcriptional activator. Recognizes and binds to the consensus octamer binding site 5'-ATAATTAA-3' in promoter of target genes. Plays a fundamental role in the gene regulatory network essential for retinal ganglion cell (RGC) differentiation. Cooperates with the transcription factor POU4F2 to achieve maximal levels of expression of RGC target genes and RGC fate specification in the developing retina. Involved in the specification of motor neurons in cooperation with LHX3 and LDB1 (By similarity). Binds to insulin gene enhancer sequences (By similarity). Essential for heart development. Marker of one progenitor cell population that give rise to the outflow tract, right ventricle, a subset of left ventricular cells, and a large number of atrial cells as well, its function is required for these progenitors to contribute to the heart. Controls the expression of FGF and BMP growth factors in this cell population and is required for proliferation and survival of cells within pharyngeal foregut endoderm and adjacent splanchnic mesoderm as well as for migration of cardiac progenitors into the heart (By similarity). |
Cellular Location | Nucleus {ECO:0000250|UniProtKB:P61372}. |
Tissue Location | Expressed in subsets of neurons of the adrenal medulla and dorsal root ganglion, inner nuclear and ganglion cell layers in the retina, the pineal and some regions of the brain |
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Background
Binds and regulates the promoters of the insulin, glucagon and somatostatin genes. Involved in the specificarion of motor neurons in cooperation with LHX3 and LDB1 (By similarity).
References
Wang M.,et al.Endocrinology 134:1416-1422(1994).
Tanizawa Y.,et al.Diabetes 43:935-941(1994).
Tanizawa Y.,et al.Diabetes 43:1171-1171(1994).
Zahedi R.P.,et al.J. Proteome Res. 7:526-534(2008).
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