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TJP1 / ZO-1 Antibody (C-Terminus)
Goat Polyclonal Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, IHC-P, IF |
|---|---|
| Primary Accession | Q07157 |
| Reactivity | Human, Mouse, Rat, Monkey, Dog |
| Host | Goat |
| Clonality | Polyclonal |
| Calculated MW | 195kDa |
| Dilution | IF (1:50-1:250), IHC-P (10 µg/ml), WB (1:500-1:2000) |
| Gene ID | 7082 |
|---|---|
| Other Names | Tight junction protein ZO-1, Tight junction protein 1, Zona occludens protein 1, Zonula occludens protein 1, TJP1, ZO1 |
| Target/Specificity | Detects endogenous levels of total ZO-1 by Western blot in whole cell and tissue lysates. |
| Reconstitution & Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. |
| Precautions | TJP1 / ZO-1 Antibody (C-Terminus) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | TJP1 (HGNC:11827) |
|---|---|
| Function | TJP1, TJP2, and TJP3 are closely related scaffolding proteins that link tight junction (TJ) transmembrane proteins such as claudins, junctional adhesion molecules, and occludin to the actin cytoskeleton (PubMed:7798316, PubMed:9792688). Forms a multistranded TJP1/ZO1 condensate which elongates to form a tight junction belt, the belt is anchored at the apical cell membrane via interaction with PATJ (By similarity). The tight junction acts to limit movement of substances through the paracellular space and as a boundary between the compositionally distinct apical and basolateral plasma membrane domains of epithelial and endothelial cells. Necessary for lumenogenesis, and particularly efficient epithelial polarization and barrier formation (By similarity). Plays a role in the regulation of cell migration by targeting CDC42BPB to the leading edge of migrating cells (PubMed:21240187). Plays an important role in podosome formation and associated function, thus regulating cell adhesion and matrix remodeling (PubMed:20930113). With TJP2 and TJP3, participates in the junctional retention and stability of the transcription factor DBPA, but is not involved in its shuttling to the nucleus (By similarity). May play a role in mediating cell morphology changes during ameloblast differentiation via its role in tight junctions (By similarity). |
| Cellular Location | Cell membrane; Peripheral membrane protein; Cytoplasmic side. Cell junction, tight junction. Cell junction. Cell junction, gap junction. Cell projection, podosome. Note=Moves from the cytoplasm to the cell membrane concurrently with cell-cell contact (PubMed:7798316). Forms a condensed tight junction-linked belt of protein during junction formation which becomes anchored to the apical cell membrane via interaction with PATJ (By similarity). At podosomal sites, is predominantly localized in the ring structure surrounding the actin core (PubMed:20930113). Colocalizes with SPEF1 at sites of cell- cell contact in intestinal epithelial cells (PubMed:31473225) {ECO:0000250|UniProtKB:O97758, ECO:0000269|PubMed:20930113, ECO:0000269|PubMed:31473225, ECO:0000269|PubMed:7798316} |
| Tissue Location | The alpha-containing isoform is found in most epithelial cell junctions. The short isoform is found both in endothelial cells and the highly specialized epithelial junctions of renal glomeruli and Sertoli cells of the seminiferous tubules |
| Volume | 100 µl |
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Provided below are standard protocols that you may find useful for product applications.
Background
The N-terminal may be involved in transducing a signal required for tight junction assembly, while the C-terminal may have specific properties of tight junctions. The alpha domain might be involved in stabilizing junctions. Plays a role in the regulation of cell migration by targeting CDC42BPB to the leading edge of migrating cells.
References
Willott E.,et al.Proc. Natl. Acad. Sci. U.S.A. 90:7834-7838(1993).
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Zody M.C.,et al.Nature 440:671-675(2006).
Cohen C.J.,et al.Proc. Natl. Acad. Sci. U.S.A. 98:15191-15196(2001).
D'Atri F.,et al.J. Biol. Chem. 277:27757-27764(2002).
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