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UNG / Uracil DNA Glycosylase Antibody (N-Terminus)
Rabbit Polyclonal Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, IHC-P, IF, E |
|---|---|
| Primary Accession | P13051 |
| Reactivity | Human, Mouse |
| Host | Rabbit |
| Clonality | Polyclonal |
| Calculated MW | 35kDa |
| Dilution | IF (20 µg/ml), IHC-P (10 µg/ml), WB (1-2 µg/ml) |
| Gene ID | 7374 |
|---|---|
| Other Names | Uracil-DNA glycosylase {ECO:0000255|HAMAP-Rule:MF_03166}, UDG {ECO:0000255|HAMAP-Rule:MF_03166}, 3.2.2.27 {ECO:0000255|HAMAP-Rule:MF_03166}, UNG {ECO:0000255|HAMAP-Rule:MF_03166} |
| Target/Specificity | At least two isoforms of UNG2 are known to exist; this antibody will only detect the longer isoform. UNG2 antibody is predicted to not cross-react with UNG1. |
| Reconstitution & Storage | Long term: -20°C; Short term: +4°C. Avoid repeat freeze-thaw cycles. |
| Precautions | UNG / Uracil DNA Glycosylase Antibody (N-Terminus) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | UNG {ECO:0000255|HAMAP-Rule:MF_03166} |
|---|---|
| Function | Uracil-DNA glycosylase that hydrolyzes the N-glycosidic bond between uracil and deoxyribose in single- and double-stranded DNA (ssDNA and dsDNA) to release a free uracil residue and form an abasic (apurinic/apyrimidinic; AP) site. Excises uracil residues arising as a result of misincorporation of dUMP residues by DNA polymerase during replication or due to spontaneous or enzymatic deamination of cytosine (PubMed:12958596, PubMed:15967827, PubMed:17101234, PubMed:22521144, PubMed:7671300, PubMed:8900285, PubMed:9016624, PubMed:9776759). Mediates error-free base excision repair (BER) of uracil at replication forks. According to the model, it is recruited by PCNA to S-phase replication forks to remove misincorporated uracil at U:A base mispairs in nascent DNA strands. Via trimeric RPA it is recruited to ssDNA stretches ahead of the polymerase to allow detection and excision of deaminated cytosines prior to replication. The resultant AP sites temporarily stall replication, allowing time to repair the lesion (PubMed:22521144). Mediates mutagenic uracil processing involved in antibody affinity maturation. Processes AICDA-induced U:G base mispairs at variable immunoglobulin (Ig) regions leading to the generation of transversion mutations (PubMed:12958596). Operates at switch sites of Ig constant regions where it mediates Ig isotype class switch recombination. Excises AICDA-induced uracil residues forming AP sites that are subsequently nicked by APEX1 endonuclease. The accumulation of staggered nicks in opposite strands results in double strand DNA breaks that are finally resolved via non-homologous end joining repair pathway (By similarity) (PubMed:12958596). |
| Cellular Location | [Isoform 1]: Mitochondrion |
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Provided below are standard protocols that you may find useful for product applications.
Background
Excises uracil residues from the DNA which can arise as a result of misincorporation of dUMP residues by DNA polymerase or due to deamination of cytosine.
References
Olsen L.C.,et al.EMBO J. 8:3121-3125(1989).
Haug T.,et al.FEBS Lett. 353:180-184(1994).
Nilsen H.,et al.Nucleic Acids Res. 25:750-755(1997).
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Mural R.J.,et al.Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
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