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SAG / Arrestin Antibody (clone S128)
Mouse Monoclonal Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, IHC-P, IF |
|---|---|
| Primary Accession | P10523 |
| Other Accession | 6295 |
| Reactivity | Human, Mouse, Rat, Pig, Bovine |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1,k |
| Clone Names | S128 |
| Calculated MW | 45120 Da |
| Dilution | IF (1:1000), IHC-P (1:100), WB (1:5000), |
| Gene ID | 6295 |
|---|---|
| Other Names | SAG, 48 kDa protein, Arrestin 1, ARRESTIN, S-arrestin, S-AG, Retinal S-antigen, Rod photoreceptor arrestin, S-antigen, RP47 |
| Target/Specificity | Clone S128 is known to react with visual arrestin from human, bovine, mouse, pig and rat. |
| Reconstitution & Storage | PBS, 10 mM sodium azide. +4°C or -20°C, Avoid repeated freezing and thawing. |
| Precautions | SAG / Arrestin Antibody (clone S128) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | SAG |
|---|---|
| Function | Binds to photoactivated, phosphorylated RHO and terminates RHO signaling via G-proteins by competing with G-proteins for the same binding site on RHO (By similarity). May play a role in preventing light-dependent degeneration of retinal photoreceptor cells (PubMed:9565049). |
| Cellular Location | Cell projection, cilium, photoreceptor outer segment. Membrane {ECO:0000250|UniProtKB:P20443}; Peripheral membrane protein {ECO:0000250|UniProtKB:P20443}. Note=Highly expressed in photoreceptor outer segments in light-exposed retina. Evenly distributed throughout rod photoreceptor cells in dark-adapted retina (By similarity) Predominantly dectected at the proximal region of photoreceptor outer segments, near disk membranes (PubMed:3720866) {ECO:0000250|UniProtKB:P08168, ECO:0000269|PubMed:3720866} |
| Tissue Location | Detected in retina, in the proximal portion of the outer segment of rod photoreceptor cells (at protein level) |
| Volume | 50 µl |
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Provided below are standard protocols that you may find useful for product applications.
Background
Arrestin is one of the major proteins of the ros (retinal rod outer segments); it binds to photoactivated- phosphorylated rhodopsin, thereby apparently preventing the transducin-mediated activation of phosphodiesterase.
References
Yamaki K.,et al.FEBS Lett. 234:39-43(1988).
Yamaki K.,et al.FEBS Lett. 236:507-507(1988).
Yamamoto S.,et al.Nat. Genet. 15:175-178(1997).
Hillier L.W.,et al.Nature 434:724-731(2005).
Roni V.,et al.BMC Genomics 8:42-42(2007).
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