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GPI Antibody (clone 4B11)
Mouse Monoclonal Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, IHC-P, IF |
|---|---|
| Primary Accession | P06744 |
| Other Accession | 2821 |
| Reactivity | Human |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Clone Names | 4B11 |
| Calculated MW | 63147 Da |
| Dilution | IF (1:100), IHC-P (10 µg/ml), WB (1:2000), |
| Gene ID | 2821 |
|---|---|
| Other Names | GPI, AMF, Autocrine motility factor, Glucose phosphate isomerase, Hexose monophosphate isomerase, Hexosephosphate isomerase, Neuroleukin, Oxoisomerase, Phosphoglucose isomerase, Phosphohexomutase, Phosphohexose isomerase, SA36, PGI, SA-36, Glucose-6- ... |
| Target/Specificity | Human GPI |
| Reconstitution & Storage | PBS, pH 7.3, 1% BSA, 50% glycerol, 0.02% sodium azide. Store at -20°C. Minimize freezing and thawing. |
| Precautions | GPI Antibody (clone 4B11) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | GPI {ECO:0000303|PubMed:2387591, ECO:0000312|HGNC:HGNC:4458} |
|---|---|
| Function | Isomerase that catalyzes the conversion of alpha-D-glucose-6- phosphate to beta-D-fructose-6-phosphate, the second step in glycolysis, and the reverse reaction in gluconeogenesis, within the cytoplasm (PubMed:28803808). Also shows C2-epimerase activity, interconverting D-glucose-6-phosphate (G6P) and D-mannose-6-phosphate (M6P) (By similarity). Also displays anomerase activity, interconverting alpha and beta-anomeric forms of G6P, D-fructose-6- phosphate and M6P (By similarity). In addition to its metabolic role, this enzyme functions extracellularly as a cytokine: acts as autocrine motility factor (AMF), a secreted angiogenic factor that enhances endothelial cell motility (PubMed:11437381). Functions as neuroleukin, a neurotrophic factor supporting the survival of spinal and sensory neurons (PubMed:11004567, PubMed:3352745). Released by lectin- stimulated T-cells to induce immunoglobulin secretion (PubMed:11004567, PubMed:3352745). |
| Cellular Location | Cytoplasm. Secreted |
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
Background
Besides it's role as a glycolytic enzyme, mammalian GPI can function as a tumor-secreted cytokine and an angiogenic factor (AMF) that stimulates endothelial cell motility. GPI is also a neurotrophic factor (Neuroleukin) for spinal and sensory neurons.
References
Gurney M.E.,et al.Submitted (MAR-1987) to the EMBL/GenBank/DDBJ databases.
Yakirevich E.,et al.Biol. Reprod. 62:1016-1023(2000).
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Grimwood J.,et al.Nature 428:529-535(2004).
Walker J.I.H.,et al.Genomics 7:638-643(1990).
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