SKIV2L / SKI2 Antibody (N-Terminus)
Rabbit Polyclonal Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| WB, IHC-P, IF, E |
---|---|
Primary Accession | Q15477 |
Other Accession | 6499 |
Reactivity | Human, Mouse, Rat |
Host | Rabbit |
Clonality | Polyclonal |
Calculated MW | 137755 Da |
Dilution | IF (20 µg/ml), IHC-P (10 µg/ml), WB (1 - 2 µg/ml), |
Gene ID | 6499 |
---|---|
Other Names | SKIV2L, 170A, DDX13, Helicase SKI2W, HLP, SKI2, SKI2W, SKIV2, Helicase-like protein, THES2 |
Target/Specificity | SKI2W antibody is human, mouse and rat reactive. At least two isoforms of SKI2W are known to exist; this antibody will detect both isoforms. |
Reconstitution & Storage | PBS, 0.02% sodium azide. Long term: -20°C; Short term: +4°C. Avoid repeat freeze-thaw cycles. |
Precautions | SKIV2L / SKI2 Antibody (N-Terminus) is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | SKIC2 (HGNC:10898) |
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Function | Helicase component of the SKI complex, a multiprotein complex that assists the RNA-degrading exosome during the mRNA decay and quality-control pathways (PubMed:16024656, PubMed:32006463, PubMed:35120588). The SKI complex catalyzes mRNA extraction from 80S ribosomal complexes in the 3'-5' direction and channels mRNA to the cytosolic exosome for degradation (PubMed:32006463, PubMed:35120588). SKI-mediated extraction of mRNA from stalled ribosomes allow binding of the Pelota-HBS1L complex and subsequent ribosome disassembly by ABCE1 for ribosome recycling (PubMed:32006463). In the nucleus, the SKI complex associates with transcriptionally active genes in a manner dependent on PAF1 complex (PAF1C) (PubMed:16024656). |
Cellular Location | Nucleus. Cytoplasm |
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Provided below are standard protocols that you may find useful for product applications.
Background
Helicase; has ATPase activity. Component of the SKI complex which is thought to be involved in exosome-mediated RNA decay and associates with transcriptionally active genes in a manner dependent on PAF1 complex (PAF1C).
References
Lee S.-G.,et al.Genomics 25:660-666(1995).
Dangel A.W.,et al.Nucleic Acids Res. 23:2120-2126(1995).
Albertella M.R.,et al.Genomics 36:240-251(1996).
Rowen L.,et al.Submitted (SEP-1997) to the EMBL/GenBank/DDBJ databases.
Mungall A.J.,et al.Nature 425:805-811(2003).
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