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Myc Tag Antibody

Purified Mouse Monoclonal Antibody (Mab)

     
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  • WB - Myc Tag Antibody AM1007a
    Western blot analysis of 12-tag protein lysate, using Myc Tag Antibody(Cat. #AM1007A). AM1007A was diluted at 1:2000. A goat anti-mouse IgG H&L(HRP) at 1:3000 dilution was used as the secondary antibody. Lysate at 20ug.
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  • WB - Myc Tag Antibody AM1007a
    Anti-Myc Tag Antibody at 1:2000 dilution + 12 tag recombinant protein lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 45-50 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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  • SPECIFICATION
  • CITATIONS: 19
  • PROTOCOLS
  • BACKGROUND
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, E
Host Mouse
Clonality Monoclonal
Isotype Mouse IgG1
Clone/Animal Names 9E10
Additional Information
Other Names Tag from c-Myc protein
Target/Specificity KLH conjugated synthetic peptide encoding c-Myc tag (AEEQKLISEEDLLRKRREQLKHKLE) was used as antigen.
Dilution WB~~1:2000
Format Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsMyc Tag Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
Citations ( 0 )

Background

Epitope tags consisting of short sequences recognized by well-characterizated monoclonal antibodies have been widely used in the study of protein expression in various systems. The HA tag (YPYDVPDYA) and Myc Tag (AEEQKLISEEDLLRKRREQLKHKLE), recognized by monoclonal antibody clones 12CA5 and 9E10, respectively, are illustrative examples. Abgent's anti-Myc monoclonal antibody (Clone 9E10) provides a simple solution to detect the expression of a Myc-tagged protein in cells.

References

Kolodziej, PA and Young RA. (1991) Methods Enzymol., 194:508-19. Sells MA and Chernoff J. (1995) Gene, 152:187-9.

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$ 365.00
$ 140.00
Cat# AM1007a
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