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GST Tag Antibody
Purified Mouse Monoclonal Antibody (Mab)
- SPECIFICATION
- CITATIONS: 8
- PROTOCOLS
- BACKGROUND
Application 
| WB, E |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | Mouse IgG1 |
| Clone/Animal Names | 9AT106 |
| Calculated MW | 26000 Da |
| Other Names | Glutathione S-transferase |
|---|---|
| Target/Specificity | Purified recombinant GST fusion protein was used to produced this monoclonal antibody. |
| Dilution | WB~~1:2,000 E~~Use at an assay dependent concentration. |
| Format | Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | GST Tag Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
Provided below are standard protocols that you may find useful for product applications.
Background
Glutathione S-transferase (GST) was originally cloned from parasite Schistosoma japonicum and it is now a widely used protein fusion partner. Vectors containing GST Tag have been developed for both prokaryotic and eukaryotic systems. The GST fusion proteins are easily purified from cell lysates by affinity chromatography using Glutathione Sepharose 4B to elute out the GST fusion protein from the column with a denaturing form of glutathione. Using the Abgent anti-GST antibody provides a simple solution to detect the expression of GST fusion proteins in cells.
References
Smith, D.B. and Johnson, K.S., (1988). Gene 67, 31.
Parker, M.W. et al., (1990) J. Mol. Biol. 213, 221.
Toye, B. et al., (1990) Infect. Immun. 58, 3909.
Guan, K.L. and Dixon, J.E. (1991) Anal. Biochem. 192, 262
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