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GNPDA1 Antibody
Mouse Monoclonal Antibody (Mab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, E |
|---|---|
| Primary Accession | P46926 |
| Other Accession | NP_005462.1 |
| Reactivity | Mouse |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1,k |
| Clone/Animal Names | 237CT2.5.2 |
| Calculated MW | 32669 Da |
| Gene ID | 10007 |
|---|---|
| Other Names | Glucosamine-6-phosphate isomerase 1, Glucosamine-6-phosphate deaminase 1, GNPDA 1, GlcN6P deaminase 1, Oscillin, GNPDA1, GNPI, HLN, KIAA0060 |
| Target/Specificity | This GNPDA1 monoclonal antibody is generated from mouse immunized with GNPDA1 recombinant protein. |
| Dilution | WB~~1:100~1000 E~~Use at an assay dependent concentration. |
| Format | Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | GNPDA1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | GNPDA1 {ECO:0000303|PubMed:26887390, ECO:0000312|HGNC:HGNC:4417} |
|---|---|
| Function | Catalyzes the reversible conversion of alpha-D-glucosamine 6- phosphate (GlcN-6P) into beta-D-fructose 6-phosphate (Fru-6P) and ammonium ion, a regulatory reaction step in de novo uridine diphosphate-N-acetyl-alpha-D-glucosamine (UDP-GlcNAc) biosynthesis via hexosamine pathway. Deamination is coupled to aldo-keto isomerization mediating the metabolic flux from UDP-GlcNAc toward Fru-6P. At high ammonium level can drive amination and isomerization of Fru-6P toward hexosamines and UDP-GlcNAc synthesis (PubMed:21807125, PubMed:26887390). Has a role in fine tuning the metabolic fluctuations of cytosolic UDP-GlcNAc and their effects on hyaluronan synthesis that occur during tissue remodeling (PubMed:26887390). Seems to trigger calcium oscillations in mammalian eggs. These oscillations serve as the essential trigger for egg activation and early development of the embryo (By similarity). |
| Cellular Location | Cytoplasm {ECO:0000250|UniProtKB:O88958}. |
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Provided below are standard protocols that you may find useful for product applications.
Background
Glucosamine-6-phosphate deaminase (EC 3.5.99.6) is an allosteric enzyme that catalyzes the reversible conversion of D-glucosamine-6-phosphate into D-fructose-6-phosphate and ammonium (Arreola et al., 2003 [PubMed 12965206]).
References
Lamesch, P., et al. Genomics 89(3):307-315(2007) Arreola, R., et al. FEBS Lett. 551 (1-3), 63-70 (2003) : Zhang, J., et al. J. Cell. Biochem. 88(5):932-940(2003) Nakamura, Y., et al. Genomics 68(2):179-186(2000) Shevchenko, V., et al. Gene 216(1):31-38(1998)
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