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SPARC Antibody(C-term) Antibody(Ascites)
Mouse Monoclonal Antibody (Mab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, E |
|---|---|
| Primary Accession | P09486 |
| Other Accession | P36378, P16975, P36233, P20112, P07214, P36377, P13213, NP_003109 |
| Reactivity | Human |
| Predicted | Bovine, Chicken, Mouse, Pig, Rabbit, Rat, Xenopus |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Clone/Animal Names | 664CT7.3.1 |
| Calculated MW | 34632 Da |
| Antigen Region | 224-251 aa |
| Gene ID | 6678 |
|---|---|
| Other Names | SPARC, Basement-membrane protein 40, BM-40, Osteonectin, ON, Secreted protein acidic and rich in cysteine, SPARC, ON |
| Target/Specificity | This SPARC antibody is generated from mice immunized with a KLH conjugated synthetic peptide between 224-251 amino acids from the C-terminal region of human SPARC. |
| Dilution | WB~~1:500~1000 E~~Use at an assay dependent concentration. |
| Format | Mouse monoclonal antibody supplied in crude ascites with 0.09% (W/V) sodium azide. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | SPARC Antibody(C-term) Antibody(Ascites) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | SPARC |
|---|---|
| Synonyms | ON |
| Function | Appears to regulate cell growth through interactions with the extracellular matrix and cytokines. Binds calcium and copper, several types of collagen, albumin, thrombospondin, PDGF and cell membranes. There are two calcium binding sites; an acidic domain that binds 5 to 8 Ca(2+) with a low affinity and an EF-hand loop that binds a Ca(2+) ion with a high affinity. |
| Cellular Location | Secreted, extracellular space, extracellular matrix, basement membrane. Note=In or around the basement membrane |
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
Background
Secreted protein acidic and rich in cysteine/osteonectin/BM40, or SPARC, is a matrix-associated protein that elicits changes in cell shape, inhibits cell-cycle progression, and influences the synthesis of extracellular matrix (ECM) (Bradshaw et al., 2003 [PubMed 12721366]).
References
Romero, R., et al. Am. J. Obstet. Gynecol. 203 (4), 361 (2010) :
Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010)
Howard, C., et al. Histol. Histopathol. 25(9):1163-1169(2010)
Johnatty, S.E., et al. PLoS Genet. 6 (7), E1001016 (2010) :
Liang, J.F., et al. J. Exp. Clin. Cancer Res. 29, 71 (2010) :
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