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POU2AF1 Antibody

Mouse Monoclonal Antibody (Mab)

     
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  • WB - POU2AF1 Antibody AM2243b
    POU2AF1 Antibody (Cat. #AM2243b) western blot analysis in Daudi cell line lysates (35μg/lane).This demonstrates the POU2AF1 antibody detected the POU2AF1 protein (arrow).
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession Q16633
Reactivity Human
Host Mouse
Clonality Monoclonal
Isotype IgG1,κ
Clone Names 1170CT1.1.1
Calculated MW 27436 Da
Additional Information
Gene ID 5450
Other Names POU domain class 2-associating factor 1, B-cell-specific coactivator OBF-1, BOB-1, OCA-B, OCT-binding factor 1, POU2AF1, OBF1
Target/Specificity Purified His-tagged POU2AF1 protein was used to produced this monoclonal antibody.
Dilution WB~~1:4000
Format Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPOU2AF1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name POU2AF1 (HGNC:9211)
Function Transcriptional coactivator that specifically associates with either POU2F1/OCT1 or POU2F2/OCT2 (PubMed:7859290). It boosts the POU2F1/OCT1 mediated promoter activity and to a lesser extent, that of POU2F2/OCT2 (PubMed:7779176). It has no intrinsic DNA-binding activity. It recognizes the POU domains of POU2F1/OCT1 and POU2F2/OCT2 (PubMed:7779176). It is essential for the response of B-cells to antigens and required for the formation of germinal centers (PubMed:7623806, PubMed:7859290). Regulates IL6 expression in B cells as POU2F2/OCT2 coactivator (By similarity).
Cellular Location Nucleus.
Tissue Location B-cell specific (PubMed:7779176, PubMed:7859290). Detected in mainly in spleen, but also in thymus, periphral blood leukocyte and small intestine (PubMed:7779176, PubMed:7859290)
Research Areas
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Background

Transcriptional coactivator that specifically associates with either OCT1 or OCT2. It boosts the OCT1 mediated promoter activity and to a lesser extent, that of OCT2. It has no intrinsic DNA-binding activity. It recognizes the POU domains of OCT1 and OCT2. It is essential for the response of B-cells to antigens and required for the formation of germinal centers.

References

Strubin M., et al. Cell 80:497-506(1995).
Gstaiger M., et al. Nature 373:360-362(1995).
Galiegue-Zouitina S., et al. C. R. Acad. Sci. III, Sci. Vie 318:1125-1131(1995).
Luo Y., et al. Mol. Cell. Biol. 15:4115-4124(1995).
Ota T., et al. Nat. Genet. 36:40-45(2004).

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$ 325.00
$ 109.00
Cat# AM2243b
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