|Application ||WB, E|
|Calculated MW||27436 Da|
|Other Names||POU domain class 2-associating factor 1, B-cell-specific coactivator OBF-1, BOB-1, OCA-B, OCT-binding factor 1, POU2AF1, OBF1|
|Target/Specificity||Purified His-tagged POU2AF1 protein was used to produced this monoclonal antibody.|
|Format||Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||POU2AF1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Transcriptional coactivator that specifically associates with either POU2F1/OCT1 or POU2F2/OCT2 (PubMed:7859290). It boosts the POU2F1/OCT1 mediated promoter activity and to a lesser extent, that of POU2F2/OCT2 (PubMed:7779176). It has no intrinsic DNA-binding activity. It recognizes the POU domains of POU2F1/OCT1 and POU2F2/OCT2 (PubMed:7779176). It is essential for the response of B-cells to antigens and required for the formation of germinal centers (PubMed:7623806, PubMed:7859290). Regulates IL6 expression in B cells as POU2F2/OCT2 coactivator (By similarity).|
|Tissue Location||B-cell specific (PubMed:7779176, PubMed:7859290). Detected in mainly in spleen, but also in thymus, periphral blood leukocyte and small intestine (PubMed:7779176, PubMed:7859290)|
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Provided below are standard protocols that you may find useful for product applications.
Transcriptional coactivator that specifically associates with either OCT1 or OCT2. It boosts the OCT1 mediated promoter activity and to a lesser extent, that of OCT2. It has no intrinsic DNA-binding activity. It recognizes the POU domains of OCT1 and OCT2. It is essential for the response of B-cells to antigens and required for the formation of germinal centers.
Strubin M., et al. Cell 80:497-506(1995).
Gstaiger M., et al. Nature 373:360-362(1995).
Galiegue-Zouitina S., et al. C. R. Acad. Sci. III, Sci. Vie 318:1125-1131(1995).
Luo Y., et al. Mol. Cell. Biol. 15:4115-4124(1995).
Ota T., et al. Nat. Genet. 36:40-45(2004).
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