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Phospho-Ser40 Tyrosine Hydroxylase Antibody
Affinity purified rabbit polyclonal antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, IHC |
|---|---|
| Primary Accession | P04177 |
| Reactivity | Human, Mouse, Rat |
| Host | Rabbit |
| Clonality | polyclonal |
| Calculated MW | 60 KDa |
| Gene ID | 25085 |
|---|---|
| Gene Name | TH |
| Other Names | Tyrosine 3-monooxygenase, Tyrosine 3-hydroxylase, TH, Th |
| Target/Specificity | Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser40 conjugated to KLH. |
| Dilution | WB~~ 1:1000
IHC~~ 1:1000 |
| Format | Prepared from rabbit serum by affinity purification via sequential chromatography on phospho- and dephosphopeptide affinity columns. |
| Antibody Specificity | Specific for the ~60k tyrosine hydroxylase protein phosphorylated at Ser40. Some higher molecular weight bands may be detected by the antibody depending upon the brain region being studied, protein loads and the detection methods used. The antibody has three orders of magnitude selectivity over dephospho TH. |
| Storage | Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | Phospho-Ser40 Tyrosine Hydroxylase Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
| Shipping | Blue Ice |
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Provided below are standard protocols that you may find useful for product applications.
Background
Tyrosine hydroxylase (TH) is the rate-limiting enzyme in the synthesis of the catecholamines Dopamine and Norepinephrine. TH antibodies can therefore be used as markers for dopaminergic and noradrenergic neurons in a variety of applications including depression, schizophrenia, Parkinson’s disease and drug abuse (Kish et al., 2001; Zhu et al., 2000; Zhu et al., 1999). TH antibodies can also be used to explore basic mechanisms of dopamine and norepinephrine signaling (Witkovsky et al., 2000; Salvatore et al., 2001; Dunkley et al., 2004). The activity of TH is also regulated by phosphorylation (Haycock et al., 1982; Haycock et al., 1992; Jedynak et al., 2002). Phospho-specific antibodies for the phosphorylation sites on TH can be used to great effect in studying this regulation and in identifying the cells in which TH phosphorylation occurs.
References
Dunkley PR, Bobrovskaya L, Graham ME, Von Nagy-Felsobuki EI, Dickson PW (2004) Tyrosine hydroxylase
phosphorylation: regulation and consequences. J Neurochem 91:1025-1043. Haycock JW, Ahn NG, Cobb MH, Krebs EG (1992) ERK1 and ERK2, two microtubule-associated protein 2 kinases, mediate the phosphorylation of tyrosine hydroxylase at serine-31 in situ. Proc Natl Acad Sci (USA) 89:2365-
2369. Haycock JW, Bennett WF, George RJ, Waymire JC (1982) Multiple site phosphorylation of tyrosine hydroxylase.
Differential regulation in situ by a 8-bromo-cAMP and acetylcholine. J Biol Chem 257:13699-13703. Jedynak JP, Ali SF, Haycock JW, Hope BT (2002) Acute administration of cocaine regulates the phosphorylation of
serine-19,-31 and-40 in tyrosine hydroxylase. J Neurochem 82:382-388. Kish SJ, Kalasinsky KS, Derkach P, Schmunk GA, Guttman M, Ang L, Adams V, Furukawa Y, Haycock JW (2001)
Striatal dopaminergic and serotonergic markers in human heroin users. Neuropsychopharmacology 24:561-567.Salvatore MF, Waymire JC, Haycock JW (2001) Depolarization-stimulated catecholamine biosynthesis: involvement
of protein kinases and tyrosine hydroxylase phosphorylation sites in situ. J Neurochem 79:349-360. Witkovsky P, Gabriel R, Haycock JW, Meller E (2000) Influence of light and neural circuitry on tyrosine hydroxylase
phosphorylation in the rat retina. J Chem Neuroanat 19:105-116. Zhu MY, Klimek V, Haycock JW, Ordway GA (2000) Quantitation of tyrosine hydroxylase protein in the locus
coeruleus from postmortem human brain. J Neurosci Meth 99:37-44. Zhu MY, Klimek V, Dilley GE, Haycock JW, Stockmeier C, Overholser JC, Meltzer HY, Ordway GA (1999) Elevated levels of tyrosine hydroxylase in the locus coeruleus in major depression. Biol Psychiatry 46:1275-1286.
Douglas A. Meyer, Edmond Richer, Stanley A. Benkovic, Kanehiro Hayashi, Janice W. Kansy, Carly F. Hale, Lily Y. Moy, Yong Kim, James P. O'Callaghan, Li-Huei Tsai, Paul Greengard, Angus C. Nairn, Christopher W. Cowan, Diane B. Miller, Pietro Antich, and James A. Bibb (2008) Striatal dysregulation of Cdk5 alters locomotor responses to ocaine, motor learning, and dendritic morphology. PNAS, 105: 18561 - 18566.
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