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Beta III Tubulin Antibody

Mouse monoclonal antibody

     
  • WB - Beta III Tubulin Antibody AN1163
    Western blot of rat cortex lysate showing specific immunolabeling of the~55k beta III tubulin protein.
    detail
  • IHC - Beta III Tubulin Antibody AN1163
    Immunostaining of mouse dentate gyrus showing beta III tubulin in red and nuclei in blue.
    detail
  • SPECIFICATION
  • CITATIONS
  • PROTOCOLS
  • BACKGROUND
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC
Primary Accession Q4QRB4
Reactivity Rat
Host Mouse
Clonality monoclonal
Isotype IgG
Clone Names AA10
Calculated MW 55 KDa
Additional Information
Gene ID 246118
Gene Name TUBB3
Other Names Tubulin beta-3 chain, Neuron-specific class III beta-tubulin, Tubb3
Target/Specificity Synthetic peptide corresponding to amino acid residues specific to beta III tubulin conjugated to KLH.
Dilution WB~~ 1:10000
IHC~~ 1:1000
Format Protein G purified culture supernatant.
Antibody Specificity Specific for the ~55kDa beta III tubulin protein. This clone is similar to themonoclonal antibody Tuj1.
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsBeta III Tubulin Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
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Research Areas
Citations (0)
citation

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Background

Tubulin is the major constituent of microtubules, existing as a heterodimer of the α and β subunits. The beta III isoform of tubulin is found almost exclusively in neuronal processes of adult tissues and is therefore an excellent marker for neurons. Neuron specific, posttranslational modifications within the C-terminal domain of beta III tubulin have been shown to be developmentally regulated suggesting that they may serve to modulate the interaction of tubulin with microtubule associated proteins (Lee et al., 1990). Additionally, beta III tubulin has been found to be highly expressed in cancer cells such as small cell lung cancer, large cell neuroendocrine carcinoma and adenocarcinomas and is correlated with an increasing histological degree of malignancy (Katsetos et al., 2003)

References

Lee MK, Rebhun LI, Frankfurter A (1990) Posttranslational modification of class III beta tubulin.
Proc Natl Acad Sci USA 87(18): 7195-7199.
Katsetos CD, Herman MM, Mork SJ (2003) Class III beta tubulin in human development and
cancer. Cell Motil Cytoskeleton 55(2):77-96.

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$ 365.00
Cat# AN1163
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Availability: 7-10 days
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