|Application ||IF, WB|
|Reactivity||Bovine, Human, Mouse, Rat|
|Clone Names||3 e1|
|Calculated MW||35, 45 KDa|
|Other Names||Neuronal migration protein doublecortin, Doublin, Lissencephalin-X, Lis-X, DCX, DBCN, LISX|
|Target/Specificity||Full length recombinant human protein expressed in and purified from E. coli.|
|Dilution||WB~~ 1:5000 |
|Format||Affinity purified from tissue culture supernatant.|
|Antibody Specificity||Specific for the ~35 & 45 k doublecortin protein.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||Doublecortin (DCX) Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
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Provided below are standard protocols that you may find useful for product applications.
Doublecortin, or DCX, is a microtubule associated protein that is expressed almost exclusively in very early neuronal development (Brown et al., 2003), making it an excellent marker for developing neuronal cells. Defects in the DCX gene lead to X-linked lissencephaly which is characterized by a lack of normal folds on the surface of the brain resulting in a smooth cerebral cortex caused by abnormal migration of neurons during development (des Portes et al., 1998; Gleeson et al., 1998).
Brown JP, Couillard-Després S, Cooper-Kuhn CM, Winkler J, Aigner L, Kuhn HG (2003) Transient expression of doublecortin during adult neurogenesis. J Comp Neurol. Dec 1;467(1):1-10.
des Portes V, Pinard JM, Billuart P, Vinet MC, Koulakoff A, CarriÃ© A, Gelot A, Dupuis E, Motte J, Berwald-Netter Y, Catala M, Kahn A, Beldjord C and Chelly J. (1998) A novel CNS gene required for neuronal migration and involved in X-linked subcortical laminar heterotopia and lissencephaly syndrome. Cell 92:51-61.
Gleeson JG, Allen KM, Fox JW, Lamperti ED, Berkovic S, Scheffer I, Cooper EC, Dobyns WB, Minnerath SR, Ross ME and Walsh CA. (1998) Doublecortin, a brain-specific gene mutated in human X-linked lissencephaly and double cortex syndrome, encodes a putative signaling protein. Cell 92:63-72.
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