|Predicted||Bovine, Mouse, Monkey, Rat|
|Calculated MW||60 KDa|
|Other Names||Beclin-1, Coiled-coil myosin-like BCL2-interacting protein, Protein GT197, BECN1, GT197|
|Target/Specificity||Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser234 conjugated to KLH.|
|Format||Prepared from rabbit serum by affinity purification via sequential chromatography on phospho- and dephosphopeptide affinity columns.|
|Antibody Specificity||Specific for the ~60k beclin1 protein phosphorylated at Ser234. Immunolabeling of the beclin-1 band is decreased in the beclin-1 alanine substitution mutant. Additional bands may be observed in non-immunoprecipitated lysates.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||Phospho-Ser234 Beclin-1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
email@example.com, and receive a free "I Love Antibodies" mug.
Provided below are standard protocols that you may find useful for product applications.
Beclin-1 plays a key role in tumor suppression and autophagy. It has been suggested that autophagy proteins such as beclin-1 can inhibit tumorigenesis and that decreased expression of these proteins may contribute to the development of human malignancies (Liang et al., 1999). Beclin-1 has been shown to interact with 14-3-3 proteins through Akt-mediated phosphorylation at Ser234 (Wang et al., 2012).
Liang, XH, Jackson S, Seaman M, Brown K, Kempkes B, Hibshoosh H, Levine B. (1999) Induction of autophagy and inhibition of tumorigenesis by beclin-1. Nature 402(6762):672-676.
Richard C. Wang, Yongjie Wei, Zhenyi An, Zhongju Zou, Guanghua Xiao, Govind Bhagat, Michael White, Julia Reichelt, and Beth Levine (2012) Akt-Mediated Regulation of Autophagy and Tumorigenesis Through Beclin 1 Phosphorylation. Science 338:956-959.
If you have used an Abcepta product and would like to share how it has performed, please click on the "Submit Review" button and provide the requested information. Our staff will examine and post your review and contact you if needed.
If you have any additional inquiries please email technical services at firstname.lastname@example.org.