Phospho-Ser459 Protein Tyrosine Phosphatase H1 Antibody
Affinity purified rabbit polyclonal antibody
|Calculated MW||104 KDa|
|Other Names||Tyrosine-protein phosphatase non-receptor type 3, Protein-tyrosine phosphatase H1, PTP-H1, PTPN3, PTPH1|
|Target/Specificity||Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser459 conjugated to KLH.|
|Format||Prepared from rabbit serum by affinity purification via sequential chromatography on phospho- and dephosphopeptide affinity columns.|
|Antibody Specificity||Specific for ~104k PTPH1 protein phosphorylated at Ser459.Immunolabeling is blocked by preadsorption of the antibody with the phosphopeptide used asantigen but not by the corresponding dephosphopeptide. Immunolabeling is also completelyeliminated by treatment withλphosphatase.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||Phospho-Ser459 Protein Tyrosine Phosphatase H1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
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Provided below are standard protocols that you may find useful for product applications.
Protein-tyrosine Phosphatase H1 (PTPH1) has recently been identified as a specific p38 γ MAPK phosphatase which is mediated through PDZ interaction (Hou et al., 2010). Ras has been demonstrated to increase both p38 γ and PTPH1 protein expression, and there is a coupling of increased p38 γ and PTPH1 protein expression in primary colon canc er tissues (Hou et al., 2010). Phosphorylation of PTPH1 at Ser459 leads to PTPH1 stabilization, which plays an important role in Ras oncogenesis and stress response (Hou et al., 2012). Additionally, phosphorylati on of PTPH1 Ser459 reveals a novel mechanism by which MAPK signals through PTPH1 to regulat e cellular response (Hou et al., 2012).
Hou SW, Zhi HY, Pohl N, Loesch M, Qi XM, Li RS, Basir Z, Chen G (2010) PTPH1 Dephosphorylates and
MAPK to Increase Ras Oncogenesis through PDZ-Mediated Interation. Cancer Res
Hou SW, Suresh PS, Qi X, Lepp A, Mirza SP, Chen G (2012)
Mitogen-activated Protein Kinase Signals
through Phosphorylating Its Phosphatase PTPH1 in Regulating Ras Protein Oncogenesis and Stress
Response. J Biol Chem 287(33): 27895-905.
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