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Phospho Ser477 Che-1 (AATF) Antibody

Affinity purified rabbit polyclonal antibody

  • WB - Phospho Ser477 Che-1 (AATF) Antibody AN1243
    Western blot of HeLa lysate showing specific immunolabeling of the ~66k Che-1 protein phosphorylated at Ser477. The phosphospecificity is shown in the second lane where immunoreactivity is blocked by preadsorption with the phospho-peptide (Peptide) used as antigen but not by the dephosphopeptide (not shown).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q9NY61
Reactivity Human
Predicted Bovine, Mouse, Monkey, Rat, Xenopus
Host Rabbit
Clonality polyclonal
Calculated MW 66 KDa
Additional Information
Gene ID 26574
Gene Name AATF
Other Names Protein AATF, Apoptosis-antagonizing transcription factor, Rb-binding protein Che-1, AATF, CHE1, DED
Target/Specificity Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser477 conjugated to KLH.
Dilution WB~~ 1:1000
Format Prepared from rabbit serum by affinity purification via sequential chromatography on phospho- and dephosphopeptide affinity columns.
Antibody Specificity Specific for the ~66k Che-1 protein phosphorylated at Ser477.Immunolabeling is blocked by preadsorption of antibody with the phospho-peptide that wasused to generate the antibody but not by the corresponding dephospho-peptide.
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPhospho Ser477 Che-1 (AATF) Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
ShippingBlue Ice
Research Areas
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Che-1, also known as AATF (apoptosis-antagonizing transcription factor), is a RNA polymerase II-binding protein involved in regulating the transcription factor E2F and promoting cell cycle progression (Burgdorf et al., 2004). It has been suggested that Che-1 may act as a neuroprotective factor against Abeta-induced apoptosis by suppressing the production of reactive oxidative species (Xie et al., 2004). The checkpoint kinase Chk2 has been shown to phosphorylate Che-1 at Ser477 contributing to the maintenance of the G2/M checkpoint induced by DNA damage (Bruno et al., 2006).


Burgdorf S, Leister P, Scheidtmann KH. (2004) TSG101 interacts with apoptosis-antagonizing transcription
factor and enhances androgen receptor-mediated transcription by promoting its monoubiquitination. J Biol
Chem. 279(17):17524-34
Xie J, Guo Q (2004) AATF protects neural cells against oxidative damage induced by amyloid beta-peptide.
Neurobiol Dis. 16(1):150-7
Bruno T, De Nicola F, Iezzi S, Lecis D, D'Angelo C, Di Padova M, Corbi N, Dimiziani L, Zannini L, Jekimovs
C, Scarsella M, Porrello A, Chersi A, Crescenzi M, Leonetti C, Khanna KK, Soddu S, Floridi A, Passananti C,
Delia D, Fanciulli M. (2006) Che-1 phosphorylation by ATM/ATR and Chk2 kinases activates p53
transcription and the G2/M checkpoint. Cancer Cell. 10(6):473-86

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$ 365.00
Cat# AN1243
Availability: 7-10 days
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