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SAP49 (SF3B4) Antibody
Mouse monoclonal antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, IF |
|---|---|
| Primary Accession | Q15427 |
| Reactivity | Bovine, Human, Mouse, Rat |
| Host | Mouse |
| Clonality | monoclonal |
| Isotype | IgG2b |
| Clone Names | 3A1 |
| Calculated MW | 49 KDa |
| Gene ID | 10262 |
|---|---|
| Gene Name | SF3B4 |
| Other Names | Splicing factor 3B subunit 4, Pre-mRNA-splicing factor SF3b 49 kDa subunit, SF3b50, Spliceosome-associated protein 49, SAP 49, SF3B4, SAP49 |
| Target/Specificity | Recombinant full length human SAP49 expressed in and purified from E. Coli. |
| Dilution | WB~~ 1:2000 IF~~ 1:1000 |
| Format | Affinity purified from tissue culture supernatant. |
| Antibody Specificity | Specific for the ~49k SAP49 protein |
| Storage | Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | SAP49 (SF3B4) Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
| Shipping | Blue Ice |
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Provided below are standard protocols that you may find useful for product applications.
Background
The spliceosome associated protein of 49 kDa (SAP49), also known as SF3B4, is a widely expressed nuclear splicing factor that has been shown to have RNA binding activity as well as direct interaction with SAP145 (Champion-Arnaud and Reed 1994). The SAP145-SAP49 complex has recently been implicated in cell cycle progression as Vpr (viral protein 1 of HIV-1) has been shown to bind SAP145 thus interfering with the proper formation and functioning of the SAP145-SAP49 complex (Terada and Yasuda 2006).
References
Champion-Arnaud P
,
Reed R
. (1994) The prespliceosome compon
ents SAP 49 and SAP 145 interact in a
complex implicated in tethering U2 snRNP to the branch site.
Genes Dev.
8(16):1974-83.
Terada Y
,
Yasuda Y
. (2006) Human immunodeficiency virus type 1 Vpr induces G2 checkpoint activation
by interacting with the splicing factor SAP145.
Mol Cell Biol.
26(21):8149-58.
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