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Anti-FOX3 (NeuN) Antibody

Our Anti-FOX3 (NeuN) primary antibody from PhosphoSolutions is mouse monoclonal. It detects bovine,

     
  •  - Anti-FOX3 (NeuN) Antibody AN1387
    Western blot of rat cortical lysate showing specific immunolabeling of the ~46/48 kDa FOX3 protein.
    detail
  •  - Anti-FOX3 (NeuN) Antibody AN1387
    Immunofluorescence of a section of rat brain stem co-labeled with Anti-FOX3(cat. 583-FOX3, green, 1:1000) and Anti-MAP2 (cat. 1100-MAP2, red, 1:5000). The Anti-FOX3 specifically labels the nuclei and the proximal cytoplasm of neuronal cells while the Anti-MAP2 labels dendrites and overlaps with FOX3 labeling the perikarya of neurons. The blue is DAPI staining of nuclear DNA.
    detail
  •  - Anti-FOX3 (NeuN) Antibody AN1387
    Immunolabeling of cultured rat neurons showing strong nuclear and distal cytoplasmic labeling with anti-FOX3(cat. 583-FOX3, 1:1000, red). The complete absence of astrocyte staining is shown using anti-GFAP (cat. 621-GFAP, 1:1000, green) and nuclear staining was done with DAPI (blue).
    detail
  •  - Anti-FOX3 (NeuN) Antibody AN1387
    Cell Line: human-derived iPSC cerebral organoids. Neurofilament-H (green), NeuN (purple), DAPI (turquoise). Image courtesy of Caroline Krall, Johns Hopkins University
    detail
  •  - Anti-FOX3 (NeuN) Antibody AN1387
    Immunofluorescence of a section of rat cerebellum showing specific labeling of Neurofilament-L (cat. 1453-NFL, 1:2000, green) and labeling of FOX3/NeuN (cat. 583-FOX3, 1:5000, red). The anti-NFL strongly labels the axons of basket calls and perikarya and processes of neuronal cells. The anti-FOX3/NeuN labels the nuclei and proximal cytoplasm of neurons. The blue is DAPI staining of nuclear DNA.
    detail
  •  - Anti-FOX3 (NeuN) Antibody AN1387
    Immunofluorescence of a section of rat hippocampus showing specific labeling of UCHL1 (cat. 2060-UCHL1, 1:5000, green) in cell bodies and dendrites of neurons, and specific labeling of FOX3 (red). The blue is DAPI staining of nuclear DNA.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC
Primary Accession A6NFN3
Reactivity Bovine, Chicken
Host Mouse
Clonality Monoclonal
Isotype IgG2b
Clone Names 1B7
Calculated MW 33873 Da
Additional Information
Gene ID 146713
Other Names FLJ56884 antibody, FLJ58356 antibody, Fox-1 homolog C antibody, fox1 homolog C antibody, Fox 3 antibody, FOX3NeuN antibody, hexaribonucleotide binding protein 3 antibody, HRNBP3 antibody, NeuN antibody, NEUN antibody, neuronal nuclei antibody, Rbfox3 antibody, RFOX3_HUMAN antibody, RNA binding protein fox-1 homolog 3 antibody, RNA binding protein fox 1 homolog (C. elegans) 3 antibody
Target/Specificity FOX3, also known as NeuN and hexaribonucleotide binding protein 3, is a neuron-specific RNA binding nuclear protein involved in the regulation of pre-mRNA alternative splicing (Kim et al., 2009). FOX3 dependent alternative splicing of Numb has recently been shown to play an important role in the progression of neuronal differentiation during vertebrate development (Kim et al., 2013).
Format Protein G Purified
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsAnti-FOX3 (NeuN) Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
ShippingBlue Ice
Research Areas
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citation

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Background

FOX3, also known as NeuN and hexaribonucleotide binding protein 3, is a neuron-specific RNA binding nuclear protein involved in the regulation of pre-mRNA alternative splicing (Kim et al., 2009). FOX3 dependent alternative splicing of Numb has recently been shown to play an important role in the progression of neuronal differentiation during vertebrate development (Kim et al., 2013).

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$ 430.00
Cat# AN1387
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Availability: 7-10 days
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