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Anti-Olig2 Antibody

Our Anti-Olig2 primary antibody from PhosphoSolutions is rabbit polyclonal. It detects human, mouse,

     
  •  - Anti-Olig2 Antibody AN1501
    Immunostaining of primary rat cortical neuroepithelial cells with anti-Olig2 (cat. 1538-OLIG2, red, 1:1000). The cells were treated with basic FGF for 30 hours prior to staining (this induces Olig2 expression in these primary cells).
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  •  - Anti-Olig2 Antibody AN1501
    Western blot of neonatal rat brain lysate showing specific immunolabeling of the ~32 kDa Olig2 protein.
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  •  - Anti-Olig2 Antibody AN1501
    An illustration differentiating mouse and human oligodendrocytes in the process of glial humanization of a mouse brain model used to study HIV-1 infection. Immunoflourescence of paraffin embedded chimeric mouse brain 5- µm thick tissue section immunolabeling oligodendrocytes with anti-OLIG2 antibody (cat. 1538-OLIG2, 1:500, green) and a human specific anti-nuclei antibody(clone 3E1.3). The blue stain is DAPI. Images courtesy Larisa Poluektova, University of Nebraska Medical Center.
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  •  - Anti-Olig2 Antibody AN1501
    Immunostaining of Olig 2 in 3-month-old mouse brain Olig 2: Red; Nuclear: Blue. Image courtesy of Mengmeng Jin, Rutgers University.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC, IF
Primary Accession Q9EQW6
Reactivity Bovine
Host Rabbit
Clonality Polyclonal
Isotype IgG
Calculated MW 32407 Da
Additional Information
Gene ID 50913
Other Names Basic domain helix loop helix protein class B 1 antibody, Basic helix loop helix protein class B 1 antibody, BHLHB antibody, bHLHB1 antibody, bHLHe19 antibody, Class B basic helix loop helix protein 1 antibody, Class B basic helix-loop-helix protein 1 antibody, class E basic helix loop helix protein 19 antibody, Class E basic helix-loop-helix protein 19 antibody, Human protein kinase C binding protein RACK17 antibody, Olig2 antibody, OLIG2_HUMAN antibody, Oligo2 antibody, Oligodendrocyte lineage transcription factor 2 antibody, Oligodendrocyte specific bHLH transcription factor 2 antibody, Oligodendrocyte transcription factor 2 antibody, OTTHUMP00000067569 antibody, OTTHUMP00000067570 antibody, PRKCBP2 antibody, Protein kinase C binding protein 2 antibody, Protein kinase C binding protein RACK17 antibody, Protein kinase C-binding protein 2 antibody, Protein kinase C-binding protein RACK17 antibody, RACK17 antibody
Target/Specificity Olig2 is a transcription factor which is required for oligodendrocyte and motor neuron specification in the spinal cord, as well as for the development of somatic motor neurons in the hindbrain. Expressed in the ventral spinal cord as early as 9.5 dpc and scattered in the mantle zone, likely corresponding to oligodendrocyte progenitors migrating out from their site of origin. From 10.5 through 14.5 dpc, Olig2 is expressed in numerous cells in the ventricular and subventricular zones of the lateral and medial ganglionic eminences, suggesting that expression might not be limited to the oligodendrocytic lineage. Olig2 has further been shown to be critical in the proliferation of malignant glioma in brain tumors (Ligon et al., 2007)
Format Protein A purified
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsAnti-Olig2 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
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Background

Olig2 is a transcription factor which is required for oligodendrocyte and motor neuron specification in the spinal cord, as well as for the development of somatic motor neurons in the hindbrain. Expressed in the ventral spinal cord as early as 9.5 dpc and scattered in the mantle zone, likely corresponding to oligodendrocyte progenitors migrating out from their site of origin. From 10.5 through 14.5 dpc, Olig2 is expressed in numerous cells in the ventricular and subventricular zones of the lateral and medial ganglionic eminences, suggesting that expression might not be limited to the oligodendrocytic lineage. Olig2 has further been shown to be critical in the proliferation of malignant glioma in brain tumors (Ligon et al., 2007)

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$ 430.00
Cat# AN1501
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