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Anti-p21/CIP1/WAF1 Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, IHC, IF |
|---|---|
| Primary Accession | P38936 |
| Host | Mouse |
| Clonality | Mouse Monoclonal |
| Isotype | IgG2a |
| Clone Names | M513 |
| Calculated MW | 18119 Da |
| Gene ID | 1026 |
|---|---|
| Other Names | Cyclin-dependent kinase inhibitor 1 CDK-interacting protein 1 Melanoma differentiation-associated protein 6 MDA-6 p21 CDKN1A CAP20, CDKN1, CIP1, MDA6, PIC1, SDI1, WAF1 |
| Dilution | WB~~1:1000 IHC~~1:100~500 IF~~1:50~200 |
| Storage | Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | Anti-p21/CIP1/WAF1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
| Shipping | Blue Ice |
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Provided below are standard protocols that you may find useful for product applications.
Background
The tumor suppressor protein p21/CIP1/WAF1 acts as an inhibitor of cell cycle progression. It functions in stoichiometric relationships forming heterotrimeric complexes with cyclins and cyclin-dependent kinases. In association with CDK2 complexes, it serves to inhibit kinase activity and block progression through G1/S. However, p21 may also enhance assembly and activity in complexes of CDK4 or CDK6 and cyclin D. The carboxy-terminal region of p21 is sufficient to bind and inhibit PCNA, a subunit of DNA polymerase, and may coordinate DNA replication with cell cycle progression. Upon UV damage or during cell cycle stages when cdc2/cyclin B or CDK2/cyclin A are active, p53 is phosphorylated and upregulates p21 transcription via a p53-responsive element. Protein levels of p21 are downregulated through ubiquitination and proteasomal degradation.
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