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NKX2.5 Antibody
Purified Mouse Monoclonal Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, E |
|---|---|
| Primary Accession | P52952 |
| Reactivity | Human |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone Names | 2E1 |
| Isotype | IgG1 |
| Calculated MW | 35kDa |
| Description | NKX2.5: NK2 transcription factor related, locus 5 (Drosophila), also known as CSX. It is a homeobox-containing transcription factor. This transcription factor functions in heart formation and development. Mutations in this gene cause atrial septal defect with atrioventricular conduction defect, and also tetralogy of Fallot, which are both heart malformation diseases. Mutations in this gene can also cause congenital hypothyroidism non-goitrous type 5, a non-autoimmune condition. Alternative splicing results in multiple transcript variants. |
| Immunogen | Purified recombinant fragment of human NKX2.5 expressed in E. Coli. |
| Formulation | Antibody are purified by protein G affinity chromatography. Liquid in PBS containing 0.03% sodium azide. |
| Gene ID | 1482 |
|---|---|
| Other Names | Homeobox protein Nkx-2.5, Cardiac-specific homeobox, Homeobox protein CSX, Homeobox protein NK-2 homolog E, NKX2-5, CSX, NKX2.5, NKX2E |
| Dilution | WB~~1/500 - 1/2000 FC~~1:200~~400 ICC~~1:200~~1000 IF~~1:200~1000. |
| Storage | Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | NKX2.5 Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | NKX2-5 |
|---|---|
| Synonyms | CSX, NKX2.5, NKX2E |
| Function | Transcription factor required for the development of the heart and the spleen (PubMed:22560297). During heart development, acts as a transcriptional activator of NPPA/ANF in cooperation with GATA4 (By similarity). May cooperate with TBX2 to negatively modulate expression of NPPA/ANF in the atrioventricular canal (By similarity). Binds to the core DNA motif of NPPA promoter (PubMed:22849347, PubMed:26926761). Together with PBX1, required for spleen development through a mechanism that involves CDKN2B repression (PubMed:22560297). Positively regulates transcription of genes such as COL3A1 and MMP2, resulting in increased pulmonary endothelial fibrosis in response to hypoxia (PubMed:29899023). |
| Cellular Location | Nucleus. |
| Tissue Location | Expressed only in the heart. |
Research Areas
Citations (0)
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info@abcepta.com, and receive a free "I Love Antibodies" mug.
Application Protocols
Provided below are standard protocols that you may find useful for product applications.
References
1. Stem Cells Dev. 2005 Aug;14(4):425-39. 2. Cancer Res. 2003 Sep 1;63(17):5329-34. 3. Circ J. 2002 Jun;66(6):561-3.
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$ 325.00
Cat# AO1319a
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