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KLHL12 Antibody
Purified Mouse Monoclonal Antibody
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Application 
| WB, E |
|---|---|
| Primary Accession | Q53G59 |
| Reactivity | Human |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone Names | 2G2 |
| Isotype | IgG1 |
| Calculated MW | 63kDa |
| Description | Serves as a substrate-specific adapter for the CUL3-based ubiquitin-protein E3 ligase complex. Negatively regulates the Wnt signaling pathway via the targeted ubiquitination and subsequent proteolysis of DVL3.Tissue specificity: Highly expressed in testis and at lower levels in the submandibular salivary gland. |
| Immunogen | Purified recombinant fragment of human KLHL12 expressed in E. Coli. |
| Formulation | Ascitic fluid containing 0.03% sodium azide. |
| Gene ID | 59349 |
|---|---|
| Other Names | Kelch-like protein 12, CUL3-interacting protein 1, DKIR homolog, hDKIR, KLHL12, C3IP1 |
| Dilution | WB~~1/500 - 1/2000 |
| Storage | Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | KLHL12 Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | KLHL12 |
|---|---|
| Synonyms | C3IP1 {ECO:0000303|Ref.1} |
| Function | Substrate-specific adapter of a BCR (BTB-CUL3-RBX1) E3 ubiquitin ligase complex that acts as a negative regulator of Wnt signaling pathway and ER-Golgi transport (PubMed:22358839, PubMed:27565346). The BCR(KLHL12) complex is involved in ER-Golgi transport by regulating the size of COPII coats, thereby playing a key role in collagen export, which is required for embryonic stem (ES) cells division: BCR(KLHL12) acts by mediating monoubiquitination of SEC31 (SEC31A or SEC31B) (PubMed:22358839, PubMed:27565346). The BCR(KLHL12) complex is also involved in neural crest specification: in response to cytosolic calcium increase, interacts with the heterodimer formed with PEF1 and PDCD6/ALG-2, leading to bridge together the BCR(KLHL12) complex and SEC31 (SEC31A or SEC31B), promoting monoubiquitination of SEC31 and subsequent collagen export (PubMed:27716508). As part of the BCR(KLHL12) complex, also acts as a negative regulator of the Wnt signaling pathway by mediating ubiquitination and subsequent proteolysis of DVL3 (PubMed:16547521). The BCR(KLHL12) complex also mediates polyubiquitination of DRD4 and PEF1, without leading to degradation of these proteins (PubMed:18303015, PubMed:20100572, PubMed:27716508). |
| Cellular Location | Cytoplasmic vesicle, COPII-coated vesicle |
| Tissue Location | Ubiquitously expressed. Highly expressed in testis and at lower levels in the submandibular salivary gland |
Research Areas
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Application Protocols
Provided below are standard protocols that you may find useful for product applications.
References
1. Exp Cell Res. 2004 Oct 15;300(1):72-83. 2. Gene. 1994 Jan 28;138(1-2):171-4.
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$ 325.00
Cat# AO1411a
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