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GATA5 Antibody

Purified Mouse Monoclonal Antibody

     
  • E - GATA5 Antibody AO1612a

    Red: Control Antigen (100ng);
    Purple: Antigen (10ng);
    Green: Antigen (50ng);
    Blue: Antigen (100ng);

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  • WB - GATA5 Antibody AO1612a
    Figure 1: Western blot analysis using GATA5 mAb against human GATA5 (AA: 168-391) recombinant protein. (Expected MW is 49.6 kDa)
    detail
  • WB - GATA5 Antibody AO1612a
    Figure 2: Western blot analysis using GATA5 mAb against HEK293 (1) and GATA5-hIgGFc transfected HEK293 (2) cell lysate.
    detail
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession Q9BWX5
Reactivity Human
Host Mouse
Clonality Monoclonal
Clone Names 1B11
Isotype IgG1
Calculated MW 41kDa
Description The protein encoded by this gene is a transcription factor that contains two GATA-type zinc fingers. The encoded protein is known to bind to hepatocyte nuclear factor-1alpha (HNF-1alpha), and this interaction is essential for cooperative activation of the intestinal lactase-phlorizin hydrolase promoter. In other organisms, similar proteins may be involved in the establishment of cardiac smooth muscle cell diversity.
Immunogen Purified recombinant fragment of human GATA5 expressed in E. Coli.
Formulation Ascitic fluid containing 0.03% sodium azide.
Additional Information
Gene ID 140628
Other Names Transcription factor GATA-5, GATA-binding factor 5, GATA5
Dilution E~~1/10000
WB~~1/500 - 1/2000
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsGATA5 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name GATA5 (HGNC:15802)
Function Transcription factor required during cardiovascular development (PubMed:23289003). Plays an important role in the transcriptional program(s) that underlies smooth muscle cell diversity (By similarity). Binds to the functionally important CEF-1 nuclear protein binding site in the cardiac-specific slow/cardiac troponin C transcriptional enhancer (PubMed:25543888).
Cellular Location Nucleus.
Research Areas
Citations (0)
citation

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References

1. Clin Cancer Res. 2009 Jun 15;15(12):3990-7. 2. World J Gastroenterol. 2010 Mar 14;16(10):1201-8.

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$ 385.00
Cat# AO1612a
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