ILK Antibody
Purified Mouse Monoclonal Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| WB, IHC, FC, E |
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Primary Accession | Q13418 |
Reactivity | Human, Mouse, Monkey |
Host | Mouse |
Clonality | Monoclonal |
Clone Names | 3A9 |
Isotype | IgG1 |
Calculated MW | 51.4kDa |
Description | Transduction of extracellular matrix signals through integrins influences intracellular and extracellular functions, and appears to require interaction of integrin cytoplasmic domains with cellular proteins. Integrin-linked kinase (ILK), interacts with the cytoplasmic domain of beta-1 integrin. This gene encodes a serine/threonine protein kinase with 4 ankyrin-like repeats, which associates with the cytoplasmic domain of beta integrins and acts as a proximal receptor kinase regulating integrin-mediated signal transduction. Multiple alternatively spliced transcript variants encoding the same protein have been found for this gene. |
Immunogen | Purified recombinant fragment of human ILK (AA: 97-244) expressed in E. Coli. |
Formulation | Ascitic fluid containing 0.03% sodium azide. |
Gene ID | 3611 |
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Other Names | Integrin-linked protein kinase, 2.7.11.1, 59 kDa serine/threonine-protein kinase, ILK-1, ILK-2, p59ILK, ILK, ILK1, ILK2 |
Dilution | E~~1/10000 WB~~1/500 - 1/2000 FC~~1/200 - 1/400 IHC~~1/200 - 1/1000 |
Storage | Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Precautions | ILK Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | ILK (HGNC:6040) |
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Function | Receptor-proximal protein kinase regulating integrin-mediated signal transduction (PubMed:8538749, PubMed:9736715). May act as a mediator of inside-out integrin signaling (PubMed:10712922). Focal adhesion protein part of the complex ILK-PINCH (PubMed:10712922). This complex is considered to be one of the convergence points of integrin- and growth factor-signaling pathway (PubMed:10712922). Could be implicated in mediating cell architecture, adhesion to integrin substrates and anchorage-dependent growth in epithelial cells (PubMed:10712922). Regulates cell motility by forming a complex with PARVB (PubMed:32528174). Phosphorylates beta-1 and beta-3 integrin subunit on serine and threonine residues, but also AKT1 and GSK3B (PubMed:8538749, PubMed:9736715). |
Cellular Location | Cell junction, focal adhesion. Cell membrane; Peripheral membrane protein; Cytoplasmic side. Cell projection, lamellipodium {ECO:0000250|UniProtKB:O55222}. Cytoplasm, myofibril, sarcomere |
Tissue Location | Highly expressed in heart followed by skeletal muscle, pancreas and kidney. Weakly expressed in placenta, lung and liver |
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Provided below are standard protocols that you may find useful for product applications.
References
1.Surgery. 2011 Aug;150(2):162-8.2.Int J Cancer. 2012 Feb 1;130(3):521-31.
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