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JAK2 Antibody

Purified Mouse Monoclonal Antibody

     
  • E - JAK2 Antibody AO2012a

    Black line: Control Antigen (100 ng);
    Purple line: Antigen(10ng);
    Blue line: Antigen (50 ng);
    Red line: Antigen (100 ng);

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  • WB - JAK2 Antibody AO2012a
    Figure 1: Western blot analysis using JAK2 mAb against human JAK2(AA: 745-955) recombinant protein. (Expected MW is 27.1 kDa)
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  • WB - JAK2 Antibody AO2012a
    Figure 2: Western blot analysis using JAK2 mAb against HEK293 (1) and JAK2(AA: 545-1124)-hIgGFc transfected HEK293 (2) cell lysate.
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  • FC - JAK2 Antibody AO2012a
    Figure 3: Flow cytometric analysis of Hela cells using JAK2 mouse mAb (green) and negative control (red).
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  • IHC - JAK2 Antibody AO2012a
    Figure 4: Immunohistochemical analysis of paraffin-embedded ovarian cancer tissues using JAK2 mouse mAb with DAB staining.
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  • IHC - JAK2 Antibody AO2012a
    Figure 5: Immunohistochemical analysis of paraffin-embedded breast cancer tissues using JAK2 mouse mAb with DAB staining.
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  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC, FC, E
Primary Accession O60674
Reactivity Human
Host Mouse
Clonality Monoclonal
Clone Names 5C12C9
Isotype IgG1
Calculated MW 130.7kDa
Description This gene product is a protein tyrosine kinase involved in a specific subset of cytokine receptor signaling pathways. It has been found to be constituitively associated with the prolactin receptor and is required for responses to gamma interferon. Mice that do not express an active protein for this gene exhibit embryonic lethality associated with the absence of definitive erythropoiesis.
Immunogen Purified recombinant fragment of human JAK2 (AA: 745-955) expressed in E. Coli.
Formulation Purified antibody in PBS with 0.05% sodium azide
Additional Information
Gene ID 3717
Other Names Tyrosine-protein kinase JAK2, 2.7.10.2, Janus kinase 2, JAK-2, JAK2
Dilution E~~1/10000
WB~~1/500 - 1/2000
FC~~1/200 - 1/400
IHC~~1/200 - 1/1000
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsJAK2 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name JAK2 (HGNC:6192)
Function Non-receptor tyrosine kinase involved in various processes such as cell growth, development, differentiation or histone modifications. Mediates essential signaling events in both innate and adaptive immunity. In the cytoplasm, plays a pivotal role in signal transduction via its association with type I receptors such as growth hormone (GHR), prolactin (PRLR), leptin (LEPR), erythropoietin (EPOR), thrombopoietin (THPO); or type II receptors including IFN-alpha, IFN- beta, IFN-gamma and multiple interleukins (PubMed:7615558). Following ligand-binding to cell surface receptors, phosphorylates specific tyrosine residues on the cytoplasmic tails of the receptor, creating docking sites for STATs proteins (PubMed:9618263). Subsequently, phosphorylates the STATs proteins once they are recruited to the receptor. Phosphorylated STATs then form homodimer or heterodimers and translocate to the nucleus to activate gene transcription. For example, cell stimulation with erythropoietin (EPO) during erythropoiesis leads to JAK2 autophosphorylation, activation, and its association with erythropoietin receptor (EPOR) that becomes phosphorylated in its cytoplasmic domain. Then, STAT5 (STAT5A or STAT5B) is recruited, phosphorylated and activated by JAK2. Once activated, dimerized STAT5 translocates into the nucleus and promotes the transcription of several essential genes involved in the modulation of erythropoiesis. Part of a signaling cascade that is activated by increased cellular retinol and that leads to the activation of STAT5 (STAT5A or STAT5B) (PubMed:21368206). In addition, JAK2 mediates angiotensin-2-induced ARHGEF1 phosphorylation (PubMed:20098430). Plays a role in cell cycle by phosphorylating CDKN1B (PubMed:21423214). Cooperates with TEC through reciprocal phosphorylation to mediate cytokine-driven activation of FOS transcription. In the nucleus, plays a key role in chromatin by specifically mediating phosphorylation of 'Tyr-41' of histone H3 (H3Y41ph), a specific tag that promotes exclusion of CBX5 (HP1 alpha) from chromatin (PubMed:19783980).
Cellular Location Endomembrane system; Peripheral membrane protein. Cytoplasm. Nucleus
Tissue Location Ubiquitously expressed throughout most tissues.
Research Areas
Citations (0)
citation

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References

Blood. 2014 May 1;123(18):2826-37.PLoS One. 2013 May 24;8(5):e64628.

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$ 385.00
Cat# AO2012a
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