Mouse Monoclonal Antibody to UFD1L
Purified Mouse Monoclonal Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| WB, IHC, ICC, E |
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Primary Accession | Q92890 |
Reactivity | Human |
Host | Mouse |
Clonality | Monoclonal |
Clone Names | 4F11A4 |
Isotype | Mouse IgG1 |
Calculated MW | 34.5kDa |
Description | The protein encoded by this gene forms a complex with two other proteins, nuclear protein localization-4 and valosin-containing protein, and this complex is necessary for the degradation of ubiquitinated proteins. In addition, this complex controls the disassembly of the mitotic spindle and the formation of a closed nuclear envelope after mitosis. Mutations in this gene have been associated with Catch 22 syndrome as well as cardiac and craniofacial defects. Alternative splicing results in multiple transcript variants encoding different isoforms. A related pseudogene has been identified on chromosome 18.; |
Immunogen | Purified recombinant fragment of human UFD1L (AA: 208-307) expressed in E. Coli. |
Formulation | Purified antibody in PBS with 0.05% sodium azide |
Application Note | ELISA: 1/10000 WB: 1/500 - 1/2000 IHC: 1/200 - 1/1000 ICC: 1/200 - 1/1000 |
Gene ID | 7353 |
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Other Names | UFD1 |
Storage | Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Precautions | Mouse Monoclonal Antibody to UFD1L is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | UFD1 (HGNC:12520) |
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Synonyms | UFD1L |
Function | Essential component of the ubiquitin-dependent proteolytic pathway which degrades ubiquitin fusion proteins. The ternary complex containing UFD1, VCP and NPLOC4 binds ubiquitinated proteins and is necessary for the export of misfolded proteins from the ER to the cytoplasm, where they are degraded by the proteasome. The NPLOC4-UFD1- VCP complex regulates spindle disassembly at the end of mitosis and is necessary for the formation of a closed nuclear envelope. It may be involved in the development of some ectoderm-derived structures (By similarity). Acts as a negative regulator of type I interferon production via the complex formed with VCP and NPLOC4, which binds to RIGI and recruits RNF125 to promote ubiquitination and degradation of RIGI (PubMed:26471729). |
Cellular Location | Nucleus {ECO:0000250|UniProtKB:Q9ES53}. Cytoplasm, cytosol {ECO:0000250|UniProtKB:Q9ES53} |
Tissue Location | Found in adult heart, skeletal muscle and pancreas, and in fetal liver and kidney |
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Provided below are standard protocols that you may find useful for product applications.
References
1.Proc Natl Acad Sci U S A. 2011 May 31;108(22):9119-24. ; 2.Cell Biochem Funct. 2003 Sep;21(3):263-7.;
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