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> home > Products > Primary Antibodies > Antibody Collections > Viral process > Mouse Monoclonal Antibody to BUB1
Mouse Monoclonal Antibody to BUB1
Purified Mouse Monoclonal Antibody
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Application 
| WB, FC, E |
|---|---|
| Primary Accession | O43683 |
| Reactivity | Human |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone Names | 1A11C7 |
| Isotype | Mouse IgG1 |
| Calculated MW | 122.4kDa |
| Description | This gene encodes a serine/threonine-protein kinase that play a central role in mitosis. The encoded protein functions in part by phosphorylating members of the mitotic checkpoint complex and activating the spindle checkpoint. This protein also plays a role in inhibiting the activation of the anaphase promoting complex/cyclosome. This protein may also function in the DNA damage response. Mutations in this gene have been associated with aneuploidy and several forms of cancer. Alternate splicing results in multiple transcript variants.; |
| Immunogen | Purified recombinant fragment of human BUB1 (AA: 1-130) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
| Application Note | ELISA: 1/10000 WB: 1/500 - 1/2000 FCM: 1/200 - 1/400 |
| Gene ID | 699 |
|---|---|
| Other Names | BUB1A; BUB1L; Hbub1 |
| Storage | Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | Mouse Monoclonal Antibody to BUB1 is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | BUB1 |
|---|---|
| Synonyms | BUB1L |
| Function | Serine/threonine-protein kinase that performs 2 crucial functions during mitosis: it is essential for spindle-assembly checkpoint signaling and for correct chromosome alignment. Has a key role in the assembly of checkpoint proteins at the kinetochore, being required for the subsequent localization of CENPF, BUB1B, CENPE and MAD2L1. Required for the kinetochore localization of PLK1. Required for centromeric enrichment of AUKRB in prometaphase. Plays an important role in defining SGO1 localization and thereby affects sister chromatid cohesion. Promotes the centromeric localization of TOP2A (PubMed:35044816). Acts as a substrate for anaphase-promoting complex or cyclosome (APC/C) in complex with its activator CDH1 (APC/C-Cdh1). Necessary for ensuring proper chromosome segregation and binding to BUB3 is essential for this function. Can regulate chromosome segregation in a kinetochore-independent manner. Can phosphorylate BUB3. The BUB1-BUB3 complex plays a role in the inhibition of APC/C when spindle-assembly checkpoint is activated and inhibits the ubiquitin ligase activity of APC/C by phosphorylating its activator CDC20. This complex can also phosphorylate MAD1L1. Kinase activity is essential for inhibition of APC/CCDC20 and for chromosome alignment but does not play a major role in the spindle-assembly checkpoint activity. Mediates cell death in response to chromosome missegregation and acts to suppress spontaneous tumorigenesis. |
| Cellular Location | Nucleus. Chromosome, centromere, kinetochore. Note=Nuclear in interphase cells. Accumulates gradually during G1 and S phase of the cell cycle, peaks at G2/M, and drops dramatically after mitosis. Localizes to the outer kinetochore. Kinetochore localization is required for normal mitotic timing and checkpoint response to spindle damage and occurs very early in prophase. AURKB, KNL1 and INCENP are required for kinetochore localization (By similarity) |
| Tissue Location | High expression in testis and thymus, less in colon, spleen, lung and small intestine. Expressed in fetal thymus, bone marrow, heart, liver, spleen and thymus. Expression is associated with cells/tissues with a high mitotic index |
Research Areas
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Application Protocols
Provided below are standard protocols that you may find useful for product applications.
References
1.Sci Signal. 2015 Jan 6;8(358):ra1. ; 2.Horm Cancer. 2013 Apr;4(2):92-102.;
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$ 385.00
Cat# AO2419a
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