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> home > Products > Primary Antibodies > Antibody Collections > Inflammasome > Mouse Monoclonal Antibody to P2RX7
Mouse Monoclonal Antibody to P2RX7
Purified Mouse Monoclonal Antibody
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Application 
| WB, IHC, FC, E |
|---|---|
| Primary Accession | Q99572 |
| Reactivity | Human |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone Names | 7G1D6 |
| Isotype | Mouse IgG2b |
| Calculated MW | 68.6kDa |
| Description | The product of this gene belongs to the family of purinoceptors for ATP. This receptor functions as a ligand-gated ion channel and is responsible for ATP-dependent lysis of macrophages through the formation of membrane pores permeable to large molecules. Activation of this nuclear receptor by ATP in the cytoplasm may be a mechanism by which cellular activity can be coupled to changes in gene expression. Multiple alternatively spliced variants have been identified, most of which fit nonsense-mediated decay (NMD) criteria.; |
| Immunogen | Purified recombinant fragment of human P2RX7 (AA: 226-452) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
| Application Note | ELISA: 1/10000 WB: 1/500 - 1/2000 IHC: 1/200 - 1/1000 FCM: 1/200 - 1/400 |
| Gene ID | 5027 |
|---|---|
| Other Names | P2X7 |
| Dilution | WB~~1:1000 IHC~~1:100~500 FC~~1:10~50 E~~N/A |
| Storage | Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | Mouse Monoclonal Antibody to P2RX7 is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | P2RX7 |
|---|---|
| Function | ATP-gated nonselective transmembrane cation channel that requires high millimolar concentrations of ATP for activation (PubMed:17483156, PubMed:25281740, PubMed:9038151). Upon ATP binding, it rapidly opens to allow the influx of small cations Na(+) and Ca(2+), and the K(+) efflux (PubMed:17483156, PubMed:20453110, PubMed:28235784, PubMed:39262850). Also has the ability to form a large pore in the cell membrane, allowing the passage of large cationic molecules (PubMed:17483156). In microglia, may mediate NADPH transport across the plasma membrane (PubMed:39142135). In immune cells, P2RX7 acts as a molecular sensor in pathological inflammatory states by detecting and responding to high local concentrations of extracellar ATP. In microglial cells, P2RX7 activation leads to the release of pro- inflammatory cytokines, such as IL-1beta and IL-18, through the activation of the NLRP3 inflammasome and caspase-1 (PubMed:26877061). Cooperates with KCNK6 to activate NLRP3 inflammasome (By similarity). Activates death pathways leading to apoptosis and autophagy (PubMed:21821797, PubMed:23303206, PubMed:28326637). Activates death pathways leading to pyroptosis (By similarity). |
| Cellular Location | Cell membrane; Multi-pass membrane protein {ECO:0000250|UniProtKB:Q64663} |
| Tissue Location | Widely expressed with highest levels in brain and immune tissues. |
Research Areas
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Application Protocols
Provided below are standard protocols that you may find useful for product applications.
References
1.Cancer Sci. 2015 Sep;106(9):1224-31. ; 2.J Hypertens. 2013 Dec;31(12):2362-9. ;
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$ 385.00
Cat# AO2457a
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