Mouse Monoclonal Antibody to ANAPC10
Purified Mouse Monoclonal Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| WB, IHC, ICC, E |
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Primary Accession | Q9UM13 |
Reactivity | Human |
Host | Mouse |
Clonality | Monoclonal |
Clone Names | 3E9A9 |
Isotype | Mouse IgG1 |
Calculated MW | 21.3kDa |
Description | ANAPC10 is a core subunit of the anaphase-promoting complex (APC), or cyclosome, a ubiquitin protein ligase that is essential for progression through the cell cycle. APC initiates sister chromatid separation by ubiquitinating the anaphase inhibitor securin (PTTG1; MIM 604147) and triggers exit from mitosis by ubiquitinating cyclin B (CCNB1; MIM 123836), the activating subunit of cyclin-dependent kinase-1 (CDK1; MIM 116940) (summary by Wendt et al., 2001 [PubMed 11524682]).; |
Immunogen | Purified recombinant fragment of human ANAPC10 (AA: 1-185) expressed in E. Coli. |
Formulation | Purified antibody in PBS with 0.05% sodium azide |
Application Note | ELISA: 1/10000 WB: 1/500 - 1/2000 IHC: 1/200 - 1/1000 ICC: 1/200 - 1/1000 |
Gene ID | 10393 |
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Other Names | DOC1; APC10 |
Storage | Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Precautions | Mouse Monoclonal Antibody to ANAPC10 is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | ANAPC10 |
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Synonyms | APC10 |
Function | Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains. |
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Provided below are standard protocols that you may find useful for product applications.
References
1.BMC Cell Biol. 2004 May 16;5:20. ; 2.Nat Struct Biol. 2001 Sep;8(9):784-8.;
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