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Phospho-4E-BP1 (Ser65)
Purified Mouse Monoclonal Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, IHC, ICC, E |
|---|---|
| Primary Accession | Q13541 |
| Reactivity | Human |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone Names | 2D1G11 |
| Isotype | Mouse IgG1 |
| Calculated MW | 12.6kDa |
| Immunogen | Synthesized peptide of human Phospho-4E-BP1 (Ser65). |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
| Gene ID | 1978 |
|---|---|
| Other Names | EIF4EBP1; BP-1; 4EBP1; 4E-BP1; PHAS-I |
| Dilution | E~~ 1/10000 FCM~~ 1/200 - 1/400 IHC~~ 1/200 - 1/1000 |
| Storage | Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | Phospho-4E-BP1 (Ser65) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | EIF4EBP1 |
|---|---|
| Function | Repressor of translation initiation that regulates EIF4E activity by preventing its assembly into the eIF4F complex: hypophosphorylated form competes with EIF4G1/EIF4G3 and strongly binds to EIF4E, leading to repress translation. In contrast, hyperphosphorylated form dissociates from EIF4E, allowing interaction between EIF4G1/EIF4G3 and EIF4E, leading to initiation of translation. Mediates the regulation of protein translation by hormones, growth factors and other stimuli that signal through the MAP kinase and mTORC1 pathways. |
| Cellular Location | Cytoplasm. Nucleus. Note=Localization to the nucleus is unaffected by phosphorylation status. {ECO:0000250|UniProtKB:Q60876} |
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Provided below are standard protocols that you may find useful for product applications.
References
1.Sci Signal. 2015 Nov 17;8(403):ra116.2.Oncotarget. 2015 Sep 15;6(27):24092-104.
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