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ASF1B

Purified Mouse Monoclonal Antibody

     
  • E - ASF1B AO2520a
    Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
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  • WB - ASF1B AO2520a
    Figure 2:Western blot analysis using ASF1B mAb against human ASF1B (AA: 1-202) recombinant protein. (Expected MW is 48.4 kDa)
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  • WB - ASF1B AO2520a
    Figure 3:Western blot analysis using ASF1B mAb against HEK293 (1) and ASF1B (AA: 1-202)-hIgGFc transfected HEK293 (2) cell lysate.
    detail
  • WB - ASF1B AO2520a
    Figure 4:Western blot analysis using ASF1B mouse mAb against Hela (1), COS7 (2), HCT116 (3), and CHO3D10 (4) cell lysate.
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  • FCM - ASF1B AO2520a
    Figure 5:Flow cytometric analysis of K562 cells using ASF1B mouse mAb (green) and negative control (red).
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  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC, ICC, E
Primary Accession Q9NVP2
Reactivity Human, Mouse, Monkey
Host Mouse
Clonality Monoclonal
Clone Names 6G7G4
Isotype Mouse IgG1
Calculated MW 22.4kDa
Immunogen Purified recombinant fragment of human ASF1B (AA: 1-202) expressed in E. Coli.
Formulation Purified antibody in PBS with 0.05% sodium azide
Additional Information
Gene ID 55723
Other Names CIA-II
Dilution E~~ 1/10000
WB~~ 1/500 - 1/2000
FCM~~1/200 - 1/400
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsASF1B is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name ASF1B {ECO:0000303|PubMed:11470414, ECO:0000312|HGNC:HGNC:20996}
Function Histone chaperone that facilitates histone deposition and histone exchange and removal during nucleosome assembly and disassembly (PubMed:11897662, PubMed:14718166, PubMed:15664198, PubMed:16151251, PubMed:21454524, PubMed:26527279). Cooperates with chromatin assembly factor 1 (CAF-1) to promote replication-dependent chromatin assembly (PubMed:11897662, PubMed:14718166, PubMed:15664198, PubMed:16151251). Also involved in the nuclear import of the histone H3-H4 dimer together with importin-4 (IPO4): specifically recognizes and binds newly synthesized histones with the monomethylation of H3 'Lys-9' (H3K9me1) and diacetylation at 'Lys-5' and 'Lys-12' of H4 (H4K5K12ac) marks in the cytosol (PubMed:20953179, PubMed:21454524, PubMed:26527279). Does not participate in replication-independent nucleosome deposition which is mediated by ASF1A and HIRA (PubMed:11897662, PubMed:14718166, PubMed:15664198, PubMed:16151251). Required for gonad development (PubMed:12842904).
Cellular Location Nucleus. Cytoplasm, cytosol
Tissue Location Highly expressed in testis and at lower levels in colon, small intestine and thymus.
Citations (0)
citation

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References

1.EMBO J. 2011 Feb 2;30(3):480-93. 2.Mol Cell Biol. 2008 Jun;28(11):3672-85.

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$ 385.00
Cat# AO2520a
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