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BCAT2

Purified Mouse Monoclonal Antibody

     
  • E - BCAT2 AO2541a
    Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
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  • WB - BCAT2 AO2541a
    Figure 2:Western blot analysis using BCAT2 mAb against human BCAT2 (AA: 259-393) recombinant protein. (Expected MW is 41.5 kDa)
    detail
  • WB - BCAT2 AO2541a
    Figure 3:Western blot analysis using BCAT2 mAb against HEK293 (1) and BCAT2 (AA: 259-393)-hIgGFc transfected HEK293 (2) cell lysate.
    detail
  • FCM - BCAT2 AO2541a
    Figure 4:Flow cytometric analysis of HeLa cells using BCAT2 mouse mAb (green) and negative control (red).
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  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC, ICC, E
Primary Accession O15382
Reactivity Human
Host Mouse
Clonality Monoclonal
Clone Names 7G3A11
Isotype Mouse IgG1
Calculated MW 44.3kDa
Immunogen Purified recombinant fragment of human BCAT2 (AA: 259-393) expressed in E. Coli.
Formulation Purified antibody in PBS with 0.05% sodium azide
Additional Information
Gene ID 587
Other Names BCAM; BCT2; PP18; BCATM
Dilution E~~ 1/10000
WB~~ 1/500 - 1/2000
FCM~~1/200 - 1/400
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsBCAT2 is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name BCAT2
Function Catalyzes the first reaction in the catabolism of the essential branched chain amino acids leucine, isoleucine, and valine (PubMed:8702755, PubMed:25653144, PubMed:17050531). May also function as a transporter of branched chain alpha-keto acids (By similarity).
Cellular Location Mitochondrion {ECO:0000250|UniProtKB:O35854}.
Tissue Location Ubiquitous..
Research Areas
Citations (0)
citation

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References

1.J Neurochem. 2012 Dec;123(6):997-1009.2.Biochemistry. 2009 Jan 27;48(3):645-56.

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$ 385.00
Cat# AO2541a
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