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CHRNA6

Purified Mouse Monoclonal Antibody

     
  • E - CHRNA6 AO2555a
    Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
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  • WB - CHRNA6 AO2555a
    Figure 2:Western blot analysis using CHRNA6 mAb against human CHRNA6 (AA: 26-239) recombinant protein. (Expected MW is 51.2 kDa)
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  • WB - CHRNA6 AO2555a
    Figure 3:Western blot analysis using CHRNA6 mAb against HEK293 (1) and CHRNA6 (AA: 26-239)-hIgGFc transfected HEK293 (2) cell lysate.
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  • FCM - CHRNA6 AO2555a
    Figure 5:Flow cytometric analysis of SH-SY5Y cells using CHRNA6 mouse mAb (green) and negative control (red).
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  • FCM - CHRNA6 AO2555a
    Figure 6:Flow cytometric analysis of SK-N-SH cells using CHRNA6 mouse mAb (green) and negative control (red).
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  • ICC - CHRNA6 AO2555a
    Figure 4:Immunofluorescence analysis of Hela cells using CHRNA6 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
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  • IHC - CHRNA6 AO2555a
    Figure 7:Immunohistochemical analysis of paraffin-embedded bladder cancer tissues using CHRNA6 mouse mAb with DAB staining.
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  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC, ICC, E
Primary Accession Q15825
Reactivity Human
Host Mouse
Clonality Monoclonal
Clone Names 5B6G8
Isotype Mouse IgG1
Calculated MW 57kDa
Immunogen Purified recombinant fragment of human CHRNA6 (AA: 26-239) expressed in E. Coli.
Formulation Purified antibody in PBS with 0.05% sodium azide
Additional Information
Gene ID 8973
Other Names CHNRA6
Dilution E~~ 1/10000
WB~~ 1/500 - 1/2000
FCM~~1/200 - 1/400
ICC~~ 1/100 - 1/500
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsCHRNA6 is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name CHRNA6
Function After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane.
Cellular Location Postsynaptic cell membrane; Multi-pass membrane protein. Cell membrane; Multi-pass membrane protein
Research Areas
Citations (0)
citation

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References

1.Mol Brain. 2014 May 2;7:35. 2.Mol Psychiatry. 2010 Jan;15(1):6-8.

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$ 385.00
Cat# AO2555a
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