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GRM3

Purified Mouse Monoclonal Antibody

     
  • E - GRM3 AO2585a
    Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
    detail
  • WB - GRM3 AO2585a
    Figure 2:Western blot analysis using GRM3 mAb against human GRM3 (AA: extra 433-576) recombinant protein. (Expected MW is 42.4 kDa)
    detail
  • WB - GRM3 AO2585a
    Figure 3:Western blot analysis using GRM3 mAb against HEK293 (1) and GRM3 (AA: extra 433-576)-hIgGFc transfected HEK293 (2) cell lysate.
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  • FCM - GRM3 AO2585a
    Figure 5:Flow cytometric analysis of SH-SY5Y cells using GRM3 mouse mAb (green) and negative control (red).
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  • ICC - GRM3 AO2585a
    Figure 4:Immunofluorescence analysis of Hela cells using GRM3 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
    detail
  • IHC - GRM3 AO2585a
    Figure 6:Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using GRM3 mouse mAb with DAB staining.
    detail
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC, ICC, E
Primary Accession Q14832
Reactivity Human
Host Mouse
Clonality Monoclonal
Clone Names 7A5A6
Isotype Mouse IgG2a
Calculated MW 99kDa
Immunogen Purified recombinant fragment of human GRM3 (AA: extra 433-576) expressed in E. Coli.
Formulation Purified antibody in PBS with 0.05% sodium azide
Additional Information
Gene ID 2913
Other Names GLUR3; mGlu3; GPRC1C; MGLUR3
Dilution E~~ 1/10000
WB~~ 1/500 - 1/2000
FCM~~1/200 - 1/400
ICC~~ 1/200 - 1/1000
IHC~~ 1/200 - 1/1000
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsGRM3 is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name GRM3
Synonyms GPRC1C, MGLUR3
Function G-protein coupled receptor for glutamate. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors. Signaling inhibits adenylate cyclase activity.
Cellular Location Cell membrane; Multi-pass membrane protein
Tissue Location Detected in brain cortex, thalamus, subthalamic nucleus, substantia nigra, hypothalamus, hippocampus, corpus callosum, caudate nucleus and amygdala.
Research Areas
Citations (0)
citation

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References

1.Prog Neuropsychopharmacol Biol Psychiatry. 2015 Oct 1;62:14-21.2.Cell Mol Biol (Noisy-le-grand). 2014 Sep 6;60(2):42-9.

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$ 385.00
Cat# AO2585a
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