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CHRNB2

Purified Mouse Monoclonal Antibody

     
  • E - CHRNB2 AO2598a
    Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
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  • WB - CHRNB2 AO2598a
    Figure 2:Western blot analysis using CHRNB2 mAb against human CHRNB2 (AA: extra 26-233) recombinant protein. (Expected MW is 51 kDa)
    detail
  • WB - CHRNB2 AO2598a
    Figure 3:Western blot analysis using CHRNB2 mAb against HEK293 (1) and CHRNB2 (AA: extra 26-233)-hIgGFc transfected HEK293 (2) cell lysate.
    detail
  •  - CHRNB2 AO2598a
    Figure 4:Flow cytometric analysis of SH-SY5Y cells using CHRNB2 mouse mAb (green) and negative control (red).
    detail
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, IHC, ICC, E
Primary Accession P17787
Reactivity Human
Host Mouse
Clonality Monoclonal
Clone Names 2D12A2
Isotype Mouse IgG2a
Calculated MW 57kDa
Immunogen Purified recombinant fragment of human CHRNB2 (AA: extra 26-233) expressed in E. Coli.
Formulation Purified antibody in PBS with 0.05% sodium azide
Additional Information
Gene ID 1141
Other Names EFNL3; nAChRB2
Dilution WB~~ 1/500 - 1/2000
IHC~~1:100~500
ICC~~N/A
E~~ 1/10000
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsCHRNB2 is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name CHRNB2 (HGNC:1962)
Function Component of neuronal acetylcholine receptors (nAChRs) that function as pentameric, ligand-gated cation channels with high calcium permeability among other activities. nAChRs are excitatory neurotrasnmitter receptors formed by a collection of nAChR subunits known to mediate synaptic transmission in the nervous system and the neuromuscular junction. Each nAchR subunit confers differential attributes to channel properties, including activation, deactivation and desensitization kinetics, pH sensitivity, cation permeability, and binding to allosteric modulators (PubMed:22361591, PubMed:27698419, PubMed:29720657, PubMed:38454578). CHRNB2 forms heteropentameric neuronal acetylcholine receptors with CHRNA2, CHRNA3, CHRNA4 and CHRNA6, as well as CHRNA5 and CHRNB3 as accesory subunits (PubMed:16835356, PubMed:20881005, PubMed:22361591, PubMed:27698419, PubMed:29720657, PubMed:38454578, PubMed:8663494). Found in two major stoichiometric forms,(CHRNA4)3:(CHRNB2)2 and (CHRNA4)2:(CHRNB2)3, the two stoichiometric forms differ in their unitary conductance, calcium permeability, ACh sensitivity and potentiation by divalent cation (PubMed:27698419, PubMed:29720657, PubMed:38454578). Heteropentameric channels with CHRNA6 and CHRNA4 exhibit high sensitivity to ACh and nicotine and are predominantly expressed in only a few brain areas, including dopaminergic neurons, norepirephrine neurons and cells of the visual system. nAChrs containing CHRNA6 subunits mediate endogenous cholinergic modulation of dopamine and gamma-aminobutyric acid (GABA) release in response to nicotine at nerve terminals (By similarity). Also forms functional nAChRs with other subunits such as CHRNA7:CHRNB2, mainly expressed in basal forebrain cholinergic neurons (PubMed:33239400, PubMed:38161283).
Cellular Location Synaptic cell membrane {ECO:0000250|UniProtKB:P12390}; Multi-pass membrane protein. Cell membrane; Multi-pass membrane protein
Research Areas
Citations (0)
citation

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References

1.Prog Neuropsychopharmacol Biol Psychiatry. 2015 Jun 3;59:84-90.2.Cancer Epidemiol Biomarkers Prev. 2009 Oct;18(10):2608-12.

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$ 385.00
Cat# AO2598a
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