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ANGPT2 Antibody (C-term)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS: 2
- PROTOCOLS
- BACKGROUND
Application 
| WB, IHC-P, FC, E |
|---|---|
| Primary Accession | O15123 |
| Other Accession | NP_001138.1 |
| Reactivity | Human, Mouse |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 56919 Da |
| Antigen Region | 404-432 aa |
| Gene ID | 285 |
|---|---|
| Other Names | Angiopoietin-2, ANG-2, ANGPT2 |
| Target/Specificity | This ANGPT2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 404-432 amino acids from the C-terminal region of human ANGPT2. |
| Dilution | WB~~1:2000 IHC-P~~1:50~100 FC~~1:25 |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | ANGPT2 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | ANGPT2 |
|---|---|
| Function | Binds to TEK/TIE2, competing for the ANGPT1 binding site, and modulating ANGPT1 signaling (PubMed:15284220, PubMed:19116766, PubMed:19223473, PubMed:9204896). Can induce tyrosine phosphorylation of TEK/TIE2 in the absence of ANGPT1 (PubMed:15284220, PubMed:19116766, PubMed:19223473, PubMed:9204896). In the absence of angiogenic inducers, such as VEGF, ANGPT2-mediated loosening of cell-matrix contacts may induce endothelial cell apoptosis with consequent vascular regression. In concert with VEGF, it may facilitate endothelial cell migration and proliferation, thus serving as a permissive angiogenic signal (PubMed:15284220, PubMed:19116766, PubMed:19223473, PubMed:9204896). Involved in the regulation of lymphangiogenesis (PubMed:32908006). |
| Cellular Location | Secreted. |
Provided below are standard protocols that you may find useful for product applications.
Background
The protein encoded by this gene is an antagonist of angiopoietin 1 (ANGPT1) and endothelial TEK tyrosine kinase (TIE-2, TEK). The encoded protein disrupts the vascular remodeling ability of ANGPT1 and may induce endothelial cell apoptosis. Three transcript variants encoding three different isoforms have been found for this gene.
References
Morrissey, C., et al. Prostate 70(16):1799-1808(2010) Romero, R., et al. Am. J. Obstet. Gynecol. 203 (4), 361 (2010) : Bento, C.F., et al. Exp. Physiol. 95(9):955-970(2010) Vrbacky, F., et al. Hematology 15(4):210-214(2010) Chen, J., et al. Biochem. Biophys. Res. Commun. 398(2):212-216(2010)
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