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APOF Antibody (Center)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - APOF Antibody (Center) AP10107c
    Anti-APOF Antibody (Center) at 1:1000 dilution + human liver lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 35 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
    detail
  • IHC-P - APOF Antibody (Center) AP10107c
    APOF antibody (Center) (Cat. #AP10107c) immunohistochemistry analysis in formalin fixed and paraffin embedded human hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of the APOF antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
    detail
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
IHC-P, WB, E
Primary Accession Q13790
Other Accession NP_001629.1
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 35399 Da
Antigen Region 111-138 aa
Additional Information
Gene ID 319
Other Names Apolipoprotein F, Apo-F, Lipid transfer inhibitor protein, LTIP, APOF
Target/Specificity This APOF antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 111-138 amino acids from the Central region of human APOF.
Dilution WB~~1:1000
IHC-P~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsAPOF Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name APOF
Function Minor apolipoprotein that associates with LDL. Inhibits cholesteryl ester transfer protein (CETP) activity and appears to be an important regulator of cholesterol transport. Also associates to a lesser degree with VLDL, Apo-AI and Apo-AII.
Cellular Location Secreted
Tissue Location Expressed by the liver and secreted in plasma.
Research Areas
Citations (0)
citation

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Background

The product of this gene is one of the minor apolipoproteins found in plasma. This protein forms complexes with lipoproteins and may be involved in transport and/or esterification of cholesterol.

References

Ruano, G., et al. Pharmacogenomics 11(7):959-971(2010)
Mosbruger, T.L., et al. J. Infect. Dis. 201(9):1371-1380(2010)
Chen, S.N., et al. BMC Med. Genet. 10, 111 (2009) :
He, Y., et al. J. Lipid Res. 49(7):1529-1537(2008)
Morton, R.E., et al. J. Lipid Res. 49(1):127-135(2008)

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$ 182.50
$ 70.00
Cat# AP10107c
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