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ACTB Antibody
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS: 4
- PROTOCOLS
- BACKGROUND
Application 
| WB, IHC-P, FC, E |
|---|---|
| Primary Accession | P60709 |
| Other Accession | A2BDB0, P63259, P63260, P63261, Q5ZMQ2, P63258, P60711, Q6QAQ1, P60710, Q4R561, P60706, P60712, P53505, NP_001092.1, P60708, P60713 |
| Reactivity | Human |
| Predicted | Xenopus, Bovine, Chicken, Horse, Monkey, Mouse, Pig, Rat, Sheep |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 41737 Da |
| Gene ID | 60 |
|---|---|
| Other Names | Actin, cytoplasmic 1, Beta-actin, Actin, cytoplasmic 1, N-terminally processed, ACTB |
| Target/Specificity | This ACTB antibody is generated from rabbits immunized with a recombinant protein from human ACTB. |
| Dilution | WB~~1:500-1:1000 IHC-P~~1:50~100 FC~~1:10~50 |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | ACTB Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | ACTB |
|---|---|
| Function | Actin is a highly conserved protein that polymerizes to produce filaments that form cross-linked networks in the cytoplasm of cells (PubMed:29581253). Actin exists in both monomeric (G-actin) and polymeric (F-actin) forms, both forms playing key functions, such as cell motility and contraction (PubMed:29581253). In addition to their role in the cytoplasmic cytoskeleton, G- and F-actin also localize in the nucleus, and regulate gene transcription and motility and repair of damaged DNA (PubMed:29925947). Part of the ACTR1A/ACTB filament around which the dynactin complex is built. The dynactin multiprotein complex activates the molecular motor dynein for ultra-processive transport along microtubules (By similarity). |
| Cellular Location | Cytoplasm, cytoskeleton. Nucleus Note=Localized in cytoplasmic mRNP granules containing untranslated mRNAs. |
Provided below are standard protocols that you may find useful for product applications.
Background
This gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. This actin is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins.
References
Martins-de-Souza, D., et al. J Psychiatr Res 44(14):989-991(2010) Ambrosino, C., et al. Mol. Cell Proteomics 9(6):1352-1367(2010) Yoo, Y., et al. Oncogene 29(2):263-272(2010) Boratynska, A., et al. Acta Virol. 54(1):41-48(2010) Dugina, V., et al. J. Cell. Sci. 122 (PT 16), 2980-2988 (2009) :
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