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ACTB Antibody

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - ACTB Antibody AP10168a
    Western blot analysis of lysates from A431, HepG2 cell line (from left to right), using ACTB Antibody(Cat. #AP10168a). AP10168a was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20ug per lane.
    detail
  • IHC-P - ACTB Antibody AP10168a
    ACTB Antibody (Cat. #AP10168a) immunohistochemistry analysis in formalin fixed and paraffin embedded human tonsil tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the ACTB Antibody for immunohistochemistry. Clinical relevance has not been evaluated.
    detail
  • FC - ACTB Antibody AP10168a
    ACTB Antibody (Cat. #AP10168a) flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
    detail
  • SPECIFICATION
  • CITATIONS: 5
  • PROTOCOLS
  • BACKGROUND
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, FC, E
Primary Accession P60709
Other Accession A2BDB0, P63259, P63260, P63261, Q5ZMQ2, P63258, P60711, Q6QAQ1, P60710, Q4R561, P60706, P60712, P53505, NP_001092.1, P60708, P60713
Reactivity Human
Predicted Xenopus, Bovine, Chicken, Horse, Monkey, Mouse, Pig, Rat, Sheep
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 41737 Da
Additional Information
Gene ID 60
Other Names Actin, cytoplasmic 1, Beta-actin, Actin, cytoplasmic 1, N-terminally processed, ACTB
Target/Specificity This ACTB antibody is generated from rabbits immunized with a recombinant protein from human ACTB.
Dilution WB~~1:500-1:1000
IHC-P~~1:50~100
FC~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsACTB Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name ACTB
Function Actin is a highly conserved protein that polymerizes to produce filaments that form cross-linked networks in the cytoplasm of cells (PubMed:25255767, PubMed:29581253). Actin exists in both monomeric (G-actin) and polymeric (F-actin) forms, both forms playing key functions, such as cell motility and contraction (PubMed:29581253). In addition to their role in the cytoplasmic cytoskeleton, G- and F- actin also localize in the nucleus, and regulate gene transcription and motility and repair of damaged DNA (PubMed:29925947). Part of the ACTR1A/ACTB filament around which the dynactin complex is built. The dynactin multiprotein complex activates the molecular motor dynein for ultra-processive transport along microtubules (By similarity).
Cellular Location Cytoplasm, cytoskeleton. Nucleus Note=Localized in cytoplasmic mRNP granules containing untranslated mRNAs.
Research Areas
Citations ( 0 )

Background

This gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. This actin is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins.

References

Martins-de-Souza, D., et al. J Psychiatr Res 44(14):989-991(2010) Ambrosino, C., et al. Mol. Cell Proteomics 9(6):1352-1367(2010) Yoo, Y., et al. Oncogene 29(2):263-272(2010) Boratynska, A., et al. Acta Virol. 54(1):41-48(2010) Dugina, V., et al. J. Cell. Sci. 122 (PT 16), 2980-2988 (2009) :

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$ 365.00
$ 140.00
Cat# AP10168a
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