Dnmt2 Antibody
Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS: 1
- PROTOCOLS
- BACKGROUND
Application
| IHC-P, WB, E |
---|---|
Primary Accession | O14717 |
Reactivity | Human, Mouse |
Host | Rabbit |
Clonality | Polyclonal |
Isotype | Rabbit IgG |
Calculated MW | 44597 Da |
Antigen Region | 346-375 aa |
Gene ID | 1787 |
---|---|
Other Names | tRNA (cytosine(38)-C(5))-methyltransferase, DNA (cytosine-5)-methyltransferase-like protein 2, Dnmt2, DNA methyltransferase homolog HsaIIP, DNA MTase homolog HsaIIP, MHsaIIP, PuMet, TRDMT1, DNMT2 |
Target/Specificity | This Dnmt2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 346-375 amino acids from human Dnmt2. |
Dilution | WB~~1:1000 IHC-P~~1:50~100 |
Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Precautions | Dnmt2 Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | TRDMT1 |
---|---|
Synonyms | DNMT2 {ECO:0000303|PubMed:16424344} |
Function | Specifically methylates cytosine 38 in the anticodon loop of tRNA(Asp). |
Cellular Location | Cytoplasm. |
Tissue Location | Ubiquitous. Higher expression in testis, ovary and thymus and at much lower levels in spleen, prostate, colon, small intestine, and peripheral blood leukocytes |
Provided below are standard protocols that you may find useful for product applications.
Background
CpG methylation is an epigenetic modification that is important for embryonic development, imprinting, and X-chromosome inactivation. Studies in mice have demonstrated that DNA methylation is required for mammalian development. This gene encodes a protein with similarity to DNA methyltransferases, but this protein does not display methyltransferase activity. The protein strongly binds DNA, suggesting that it may mark specific sequences in the genome. Alternative splicing results in multiple transcript variants encoding different isoforms.
References
Hermann, A., et al., J. Biol. Chem. 278(34):31717-31721 (2003).
Franchina, M., et al., Int. J. Biochem. Cell Biol. 33(11):1104-1115 (2001).
Dong, A., et al., Nucleic Acids Res. 29(2):439-448 (2001).
Yoder, J.A., et al., Hum. Mol. Genet. 7(2):279-284 (1998).
Van den Wyngaert, I., et al., FEBS Lett. 426(2):283-289 (1998).
If you have used an Abcepta product and would like to share how it has performed, please click on the "Submit Review" button and provide the requested information. Our staff will examine and post your review and contact you if needed.
If you have any additional inquiries please email technical services at tech@abcepta.com.