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Dnmt3a Antibody

Purified Rabbit Polyclonal Antibody (Pab)

  • WB - Dnmt3a Antibody AP1034a
    All lanes : Anti-Dnmt3a Antibody at 1:1000 dilution Lane 1: Hela whole cell lysate Lane 2: 293 whole cell lysate Lane 3: Human skeletal muscle tissue lysate Lane 4: NIH/3T3 whole cell lysate Lane 5: Mouse brain tissue lysate Lane 6: Rat heart tissue lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 102 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • WB - Dnmt3a Antibody AP1034a
    All lanes : Anti-Dnmt3a Antibody at 1:1000 dilution Lane 1: Hela whole cell lysate Lane 2: T47D whole cell lysate Lane 3: NIH/3T3 whole cell lysate Lane 4: Rat heart tissue lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 102 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • WB - Dnmt3a Antibody AP1034a
    Western blot analysis of anti-Dnmt3a Pab (Cat. #AP1034a) in T47-D cell lysate. Dnmt3a (Arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
  • WB - Dnmt3a Antibody AP1034a
    Lysates from mice thymus tissue after radiation were subjected to WB using antibody against DNMT3a. CT, control animals; FR, animals subjected to fractionated exposure; AC, acutely exposed animals. All sample loading was normalized to protein content. Representative Western blots from three independent experiments are shown; each lane represents a protein extract of a thymus of one animal. (Mol. Cancer Res. 2005 Oct 01;3(10):553-561)
  • IHC-P - Dnmt3a Antibody AP1034a
    Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
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Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q9Y6K1
Other Accession Q1LZ53, O88508, Q4W5Z4
Reactivity Human, Mouse, Rat
Predicted Chicken
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 101858 Da
Antigen Region 457-486 aa
Additional Information
Gene ID 1788
Other Names DNA (cytosine-5)-methyltransferase 3A, Dnmt3a, DNA methyltransferase HsaIIIA, DNA MTase HsaIIIA, MHsaIIIA, DNMT3A
Target/Specificity This Dnmt3a antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 457-486 amino acids from human Dnmt3a.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsDnmt3a Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Function Required for genome-wide de novo methylation and is essential for the establishment of DNA methylation patterns during development (PubMed:12138111, PubMed:16357870, PubMed:30478443). DNA methylation is coordinated with methylation of histones (PubMed:12138111, PubMed:16357870, PubMed:30478443). It modifies DNA in a non-processive manner and also methylates non-CpG sites (PubMed:12138111, PubMed:16357870, PubMed:30478443). May preferentially methylate DNA linker between 2 nucleosomal cores and is inhibited by histone H1 (By similarity). Plays a role in paternal and maternal imprinting (By similarity). Required for methylation of most imprinted loci in germ cells (By similarity). Acts as a transcriptional corepressor for ZBTB18 (By similarity). Recruited to trimethylated 'Lys-36' of histone H3 (H3K36me3) sites (By similarity). Can actively repress transcription through the recruitment of HDAC activity (By similarity). Also has weak auto-methylation activity on Cys-710 in absence of DNA (By similarity).
Cellular Location Nucleus. Chromosome Cytoplasm. Note=Accumulates in the major satellite repeats at pericentric heterochromatin {ECO:0000250|UniProtKB:O88508}
Tissue Location Highly expressed in fetal tissues, skeletal muscle, heart, peripheral blood mononuclear cells, kidney, and at lower levels in placenta, brain, liver, colon, spleen, small intestine and lung
Research Areas
Citations ( 0 )


CpG methylation is an epigenetic modification that is important for embryonic development, imprinting, and X-chromosome inactivation. Studies in mice have demonstrated that DNA methylation is required for mammalian development. Dnmt3a is a DNA methyltransferase that is thought to function in de novo methylation, rather than maintenance methylation. The protein localizes to the cytoplasm and nucleus and its expression is developmentally regulated.


Xie, S., et al., Gene 236(1):87-95 (1999). Robertson, K.D., et al., Nucleic Acids Res. 27(11):2291-2298 (1999).

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$ 365.00
$ 140.00
Cat# AP1034a
Availability: In stock
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