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SPATC1 Antibody (N-term)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - SPATC1 Antibody (N-term) AP10622a
    All lanes : Anti-SPATC1 Antibody (N-term) at 1:1000 dilution Lane 1: HepG2 whole cell lysate Lane 2: human testis lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 62kDa Blocking/Dilution buffer: 5% NFDM/TBST.
    detail
  • WB - SPATC1 Antibody (N-term) AP10622a
    SPATC1 Antibody (N-term) (Cat. #AP10622a) western blot analysis in mouse heart tissue lysates (35ug/lane).This demonstrates the SPATC1 antibody detected the SPATC1 protein (arrow).
    detail
  • IHC-P - SPATC1 Antibody (N-term) AP10622a
    SPATC1 antibody (N-term) (Cat. #AP10622a) immunohistochemistry analysis in formalin fixed and paraffin embedded human testis carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the SPATC1 antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
    detail
  • SPECIFICATION
  • CITATIONS: 1
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
IHC-P, WB, E
Primary Accession Q76KD6
Other Accession Q148B6, NP_001127846.1, NP_940974.2
Reactivity Human, Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 62399 Da
Antigen Region 1-30 aa
Additional Information
Gene ID 375686
Other Names Speriolin, Spermatogenesis and centriole-associated protein 1, Spermatogenesis-associated protein 15, Spermatogenic cell-specific Cdc20-binding protein, SPATC1, SPATA15, SPRN
Target/Specificity This SPATC1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human SPATC1.
Dilution WB~~1:1000
IHC-P~~1:50~100
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsSPATC1 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name SPATC1
Synonyms SPATA15, SPRN
Cellular Location Cytoplasm. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome Note=Colocalizes with the centrosomal pericentrin protein PCNT1 (By similarity). Located in the connecting piece of sperm. {ECO:0000250, ECO:0000269|PubMed:20542897}
Tissue Location Detected only in testis.
Research Areas
Citations ( 0 )

References

Goto, M., et al. Hum. Reprod. 25(8):1884-1894(2010)
Goto, M., et al. J. Biol. Chem. 279(40):42128-42138(2004)

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$ 365.00
$ 140.00
Cat# AP10622a
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