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SREBF2 Antibody (Center)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, IF, IHC-P, E |
|---|---|
| Primary Accession | Q12772 |
| Other Accession | NP_004590.2 |
| Reactivity | Human |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 123688 Da |
| Antigen Region | 399-427 aa |
| Gene ID | 6721 |
|---|---|
| Other Names | Sterol regulatory element-binding protein 2, SREBP-2, Class D basic helix-loop-helix protein 2, bHLHd2, Sterol regulatory element-binding transcription factor 2, Processed sterol regulatory element-binding protein 2, SREBF2, BHLHD2, SREBP2 |
| Target/Specificity | This SREBF2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 399-427 amino acids from the Central region of human SREBF2. |
| Dilution | IF~~1:10~50 WB~~1:1000 IHC-P~~1:10~50 |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | SREBF2 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | SREBF2 {ECO:0000303|PubMed:32322062, ECO:0000312|HGNC:HGNC:11290} |
|---|---|
| Function | [Sterol regulatory element-binding protein 2]: Precursor of the transcription factor form (Processed sterol regulatory element- binding protein 2), which is embedded in the endoplasmic reticulum membrane (PubMed:32322062). Low sterol concentrations promote processing of this form, releasing the transcription factor form that translocates into the nucleus and activates transcription of genes involved in cholesterol biosynthesis (PubMed:32322062). |
| Cellular Location | [Sterol regulatory element-binding protein 2]: Endoplasmic reticulum membrane; Multi- pass membrane protein. Golgi apparatus membrane; Multi-pass membrane protein. Cytoplasmic vesicle, COPII-coated vesicle membrane; Multi-pass membrane protein. Note=At high sterol concentrations, the SCAP-SREBP is retained in the endoplasmic reticulum (PubMed:32322062). Low sterol concentrations promote recruitment into COPII-coated vesicles and transport of the SCAP-SREBP to the Golgi, where it is processed (PubMed:32322062). |
| Tissue Location | Ubiquitously expressed in adult and fetal tissues. |
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Provided below are standard protocols that you may find useful for product applications.
Background
SREBPs, including SREBP-1a, SREBP-1c, and SREBP-2, constitute a family of basic helix-loop-helix (bHLH) transcription factors that play a critical role in lipid homeostasis by regulating genes involved in cholesterol and fatty acid metabolism. Each SREBP consists of three domains, including an amino-terminal transcription factor domain of ~480 amino acids, a middle hydrophobic region of ~80 amino acids containing two hydrophobic transmembrane segments, and a carboxy-terminal regulatory domain of ~590 amino acids. SREBP-2 regulates cholesterol synthesis by activating the transcription of genes for HMG-CoA reductase and other enzymes of the cholesterol synthetic pathway. SREBP-2 is ubiquitously detected in various tissues. Under basal conditions SREBP is bound to ER membranes as a glycosylated precursor protein. Upon cholesterol depletion, the protein is cleaved to its active forms (about 50-68 kDa) and translocated into the nucleus to stimulate transcription of genes involved in the uptake and synthesis of cholesterol.
References
Gerin, I., et al. J. Biol. Chem. 285(44):33652-33661(2010) Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010) Marquart, T.J., et al. Proc. Natl. Acad. Sci. U.S.A. 107(27):12228-12232(2010) Najafi-Shoushtari, S.H., et al. Science 328(5985):1566-1569(2010) Pramfalk, C., et al. J. Lipid Res. 51(6):1354-1362(2010)
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