DHTKD1 Antibody (Center)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| WB, E |
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Primary Accession | Q96HY7 |
Other Accession | Q4KLP0, A2ATU0, NP_061176.3 |
Reactivity | Human, Mouse |
Predicted | Rat |
Host | Rabbit |
Clonality | Polyclonal |
Isotype | Rabbit IgG |
Calculated MW | 103077 Da |
Antigen Region | 392-421 aa |
Gene ID | 55526 |
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Other Names | Probable 2-oxoglutarate dehydrogenase E1 component DHKTD1, mitochondrial, Dehydrogenase E1 and transketolase domain-containing protein 1, DHTKD1, KIAA1630 |
Target/Specificity | This DHTKD1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 392-421 amino acids from the Central region of human DHTKD1. |
Dilution | WB~~1:1000 |
Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Precautions | DHTKD1 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | DHTKD1 |
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Synonyms | KIAA1630 |
Function | 2-oxoadipate dehydrogenase (E1a) component of the 2- oxoadipate dehydrogenase complex (OADHC) (PubMed:29191460, PubMed:29752936, PubMed:32303640, PubMed:32633484, PubMed:32695416). Participates in the first step, rate limiting for the overall conversion of 2-oxoadipate (alpha-ketoadipate) to glutaryl-CoA and CO(2) catalyzed by the whole OADHC (PubMed:29191460, PubMed:32695416). Catalyzes the irreversible decarboxylation of 2-oxoadipate via the thiamine diphosphate (ThDP) cofactor and subsequent transfer of the decarboxylated acyl intermediate on an oxidized dihydrolipoyl group that is covalently amidated to the E2 enzyme (dihydrolipoyllysine- residue succinyltransferase or DLST) (Probable) (PubMed:29752936, PubMed:32303640, PubMed:32633484). Can catalyze the decarboxylation of 2-oxoglutarate in vitro, but at a much lower rate than 2-oxoadipate (PubMed:29191460, PubMed:29752936, PubMed:32633484, PubMed:32695416). Responsible for the last step of L-lysine, L-hydroxylysine and L- tryptophan catabolism with the common product being 2-oxoadipate (Probable). |
Cellular Location | Mitochondrion. |
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Provided below are standard protocols that you may find useful for product applications.
Background
The 2-oxoglutarate dehydrogenase complex catalyzes the overall conversion of 2-oxoglutarate to succinyl-CoA and CO(2). It contains multiple copies of three enzymatic components: 2-oxoglutarate dehydrogenase (E1), dihydrolipoamide succinyltransferase (E2) and lipoamide dehydrogenase (E3) (By similarity).
References
Wang, A.G., et al. Biochem. Biophys. Res. Commun. 345(3):1022-1032(2006)
Grupe, A., et al. Am. J. Hum. Genet. 78(1):78-88(2006)
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